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当前位置: 首页 > 文章 > 发酵乳球菌菌株的PCR鉴定 食品科学 2007,28 (3) 206-210
Position: Home > Articles > PCR-based Methods for Identification of Coccus Strains from Fermented Milk FOOD SCIENCE 2007,28 (3) 206-210

发酵乳球菌菌株的PCR鉴定

作  者：
王庭柱;高学军;丛玉婷;杨振宇
单  位：
农垦建三江分局七星农场畜牧渔业总公司;东北农业大学教育部乳品科学重点实验室;大连水产学院生命科学与技术学院
关键词：
乳酸乳球菌;嗜热链球菌;PCR;鉴定
摘  要：
对9株发酵乳球菌标准菌株进行MRS固体平板培养基划线分离和镜检观察,采用特异性PCR对其进行鉴定,划线分离得到11株菌;依据各菌种代谢酶编码基因序列差异设计菌种特异性PCR引物,进行PCR扩增,将其在菌株水平上鉴定为9株菌(6株乳酸乳球菌、2株嗜热链球菌和1株无乳链球菌)。实验还获得了2个乳酸乳球菌的种特异性PCR引物LclamyLF-R和LclmapA2F-R、2个嗜热链球菌的种特异性PCR引物SttglgPF-R和SttamyLF-R以及可能是标准菌株AS1.1936的株特异性的PCR引物LclamyYF-R。
译  名：
PCR-based Methods for Identification of Coccus Strains from Fermented Milk
作  者：
WANG Ting-zhu1,GAO Xue-jun2,*,CONG Yu-ting3,YANG Zhen-yu2 (1.Animal Science and Fishery Head Office of Qixing Farm, Jiansanjiang Land Reclamation Bureau, Jiamusi 156300, China; 2.Key Laboratory of Dairy Science, Ministry of Education, Northeast Agricultural University, Harbin 150030, China; 3.College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China)
关键词：
Lactococcus lactis;Streptococcus thermophilus;PCR(polymase chain reaction);identification
摘  要：
In this paper, the isolation and identification of 9 lactic acid Coccus strains from fermented milk were studied. The lactic acid bacteria were isolated by MRS plate streaking cultivation and microscopic examination, and were identified by specific PCR technique. The results of plate streaking cultivation showed that there are 11 strains. The specific PCR primers were designed on the basis of biochemical metabolic enzymes genes coding, different between Lactococcus lactis and Streptococcus thermophilus at the level of strains. The PCR amplifications classify these strains into 9 strains; 6 lactococcus lactis strains, 2 Streptococcus thermophilus strains and 1 Streptococcus agalactiae respectively. In addition, 2 species-specific PCR primers of Lactococcus lactis named LclamyLF-R and LclmapA2F-R respectively were acquired, and Streptococcus thermophilus also had two primers, SttglgPF-R and SttamyLF-R respectively. And even one strain-specific primer of AS1.1936 named LclamyYF-R is obtained.

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