中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 耐酸产蛋白酶芽孢杆菌SD48菌株液体发酵豆粕产大豆肽条件的优化 河南农业科学 2019,48 (8) 140-146
Position: Home > Articles > Optimization of Liquid Fermentation Condition of Producing Soybean Peptide by Acid-resisting and Protease-producing Bacillus Strain SD48 with Soybean Meal Journal of Henan Agricultural Sciences 2019,48 (8) 140-146

耐酸产蛋白酶芽孢杆菌SD48菌株液体发酵豆粕产大豆肽条件的优化

作  者：
周星华;董伟洁;姜军坡;刘双;王世英;朱宝成
单  位：
河北农业大学生命科学学院;河北省畜牧站;河北农业大学 生命科学学院
关键词：
发酵豆粕;大豆肽;甲基营养型芽孢杆菌;抗氧化活性;液体发酵
摘  要：
为了确定甲基营养型芽孢杆菌(Bacillus methylotrophicus)SD48菌株液体发酵豆粕产大豆肽的最优培养基组成和最佳发酵条件,以大豆肽含量为指标,通过单因素试验确定最优碳源种类、无机盐种类和发酵温度;通过正交试验确定最适碳源、豆粕、硫酸铵和无机盐的含量,以及SD48菌株接种量、起始pH值、装瓶量和发酵时间.结果表明,最优培养基组成:可溶性淀粉含量为1.00%,豆粕含量为15.00%,硫酸铵含量为4.00%,硫酸锰含量为0.09%;最适发酵条件:起始pH值为6.00,装瓶量为40%,SD48菌株的接种量为6.00%,发酵温度为28℃,培养时间为72 h.由此获得的大豆肽含量为8.03%,相对含量为71.06%,抗氧化活性达到343.70μg AEAC/mL.综上,获得了SD48菌株液体发酵豆粕产大豆肽的最佳条件,提高了大豆肽的产量.
译  名：
Optimization of Liquid Fermentation Condition of Producing Soybean Peptide by Acid-resisting and Protease-producing Bacillus Strain SD48 with Soybean Meal
作  者：
ZHOU Xinghua;DONG Weijie;JIANG Junpo;LIU Shuang;WANG Shiying;ZHU Baocheng;College of Life Science,Hebei Agricultural University;College of Chemistry and Environmental Science,Hebei University;Hebei Animal Husbandry Station;
关键词：
Fermented soybean meal;;Soybean peptide;;Bacillus methylotrophicus;;Antioxidant activity;;Liquid fermentation
摘  要：
In order to determine the optimum medium composition and fermentation conditions for soybean peptide production from soybean meal by Bacillus methylotrophicus SD48 strain via liquid fermentation, with the content of soybean peptide as an index,the optimum carbon source,inorganic salt and fermentation temperature were determined by single factor test.The optimum carbon source content,soybean meal content,ammonium sulfate content,inorganic salt content,inoculation quantity of SD48 strain,initial pH value,bottling quantity and fermentation time were determined by orthogonal test.The optimal fermentation conditions were as follows:fermentation medium was composed of soluble starch 1.00%,soybean meal 15.00%,ammonium sulfate 4.00%,and manganese sulfate 0.09%,initial pH was 6.00,loading amount was 40%,inoculation amount was 6.00%,and fermented at 28 ℃ for 72 h,when soybean peptide content could reach 8.03%.Meanwhile,soybean peptide relative content and antioxidant activity could respectively reach 71.06% and 343.70 μg AEAC/mL.In conlusion,the optimum conditions for the production of soybean peptide from SD48 strain liquid fermented soybean meal were obtained,and the yield of soybean peptide was increased.

相似文章

导出引用
计量
文章访问数: 20
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46