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Position: Home > Articles > Optimization of Extraction Process for Flavonoids from Polygonum aviculare L. Based on HPLC Quantitation FOOD SCIENCE 2012,33 (14) 36-40

基于高效液相色谱法定量优化萹蓄黄酮提取工艺

作  者：
许福泉;冯媛媛;顾丽蕴
单  位：
淮海工学院电子工程学院;淮海工学院江苏省海洋生物技术重点建设实验室;淮海工学院江苏省海洋生物重点建设实验室淮海工学院电子工程学院
关键词：
黄酮;高效液相色谱;提取工艺;萹蓄
摘  要：
目的:优化萹蓄黄酮提取工艺,提高提取液中目标黄酮成分含量。方法:利用高效液相方法考查正交试验在不同粒度、料液比、乙醇体积分数、提取时间、提取温度下目标黄酮总质量浓度,从而对提取工艺进行优化。结果:原料粒度对目标黄酮总质量浓度的影响最大,最佳提取工艺为粒度40目、料液比1:30(g/mL)、提取温度80℃、提取时间2h、乙醇体积分数65%,提取液中目标黄酮总质量浓度为228.9μg/mL。
译  名：
Optimization of Extraction Process for Flavonoids from Polygonum aviculare L. Based on HPLC Quantitation
作  者：
XU Fu-quan 1,FENG Yuan-yuan 2,GU Li-yun 1(1.Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China;2.College of Electronic Engineering,Huaihai Institute of Technology,Lianyungang 222005,China)
关键词：
flavonoid;high performance liquid chromatography(HPLC);extraction process;Polygonum aviculare L.
摘  要：
Objective: To optimize the extraction condition of flavonoids from Polygonum aviculare L based on HPLC quantitation.Methods: The effects of raw material particle size,ethanol concentration,soaking(extraction) time,solid-tosolvent ratio and extraction temperature on the extraction rate of total flavonoids were explored by orthogonal array design.Results: The optimal extraction conditions were particle size of 40 mesh,solid-to-solvent ratio of 1:30(g/mL),extraction temperature of 80 ℃,extraction time of 2 h and ethanol concentration of 65%.The total flavonoids concentration of the extract obtained under these conditions was 228.9μg/mL.Among all tested process parameters,raw material particle size had the biggest effect on the extraction rate of total flavonoids.Conclusion: HPLC provides a reliable method for quantifying total flavonoids.The optimize extraction process provides a reliable,simple and useful approach for the extraction and exploitation of flavonoids from Polygonum aviculare L.

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