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当前位置: 首页 > 文章 > 貉源犬瘟热病毒的分离鉴定及其H基因的序列分析 黑龙江畜牧兽医 2013 (13) 7-9，162-163
Position: Home > Articles > Isolation and identification of canine distemper virus from raccoon dog and sequence analysis of its gene H Heilongjiang Animal Science and Veterinary Medicine 2013 (13) 7-9，162-163

貉源犬瘟热病毒的分离鉴定及其H基因的序列分析

作  者：
郭抗抗[1;闫喜军;柴秀丽;张蕾;赵建军
单  位：
中国农业科学院特产研究所
关键词：
犬瘟热病毒(CDV);分离;鉴定;H基因
摘  要：
为了研究我国貉源犬瘟热病毒(CDV)的流行、遗传变异性,试验从某养殖场选取5只疑似患犬瘟热病死貉的肝脏、肺脏、脾脏、肠等脏器,利用Vero细胞分离出1株病毒,通过免疫荧光、RT-PCR等方法鉴定为CDV,命名为HLJ(11),利用其RNA为模板,采用RT-PCR扩增血凝素(H)基因,进行序列测定与分析。结果表明:分离株HLJ(11)的H基因序列与HeB(07)1、SD(09)3、SD(07)1同源性最高,分别为99.9%、99.7%、99.6%,与Strain CDV 3、Convac vaccine strain的同源性最低,为91.4%;遗传进化分析显示,分离株HLJ(11)为Asia-Ⅰ型。
译  名：
Isolation and identification of canine distemper virus from raccoon dog and sequence analysis of its gene H
作  者：
GUO Kang-kang 1,2,YAN Xi-jun 2,CHAI Xiu-li 2,ZHANG Lei 2,ZHAO Jian-jun 2,ZHANG Hai-ling 2,BAI Xue 2,SHAO Xi-qun 2(1.College of Biological and Environmental Engineering,Jiangsu University of Science and Technology,Zhenjiang 212003,China;2.Institute of Special Animal and Plant Sciences of CAAS,Changchun 132109,China)
关键词：
canine distemper virus(CDV);isolation;identification;gene H
摘  要：
To study the prevalence and variability of canine distemper virus(CDV) from raccoon dog in China,the livers,lungs,spleens,intestines and other organs were collected from five dead raccoon dogs suspected with CDV infection in a certain farm,and then a strain of virus was isolated using vero cells culture.The virus was identified to be a canine distemper virus by immunofluorescence,RT-PCR and other methods,which was named HLJ(11).The H gene of the isolated strain was amplified by RT-PCR,and then was cloned and sequenced,taking the RNA from the isolate HLJ(11) as a template.The results showed that the homologies of nucleic acid sequences for the H gene from HLJ(11) with HeB(07) 1,SD(09) 3 and SD(07) 1 were high(99.9%,99.7%,99.6%,respectively).The homologies of nucleic acid sequences with Strain CDV 3 and Convac vaccine strain were low(91.4%).The phylogenetic tree showed that the isolated strain belonged to Asia-Ⅰ.

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