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Position: Home > Articles > Advances in Agrobacterium-mediated plant genetic transformation:the interaction between Agrobacterium infection and plant resistance response Journal of Huazhong Agricultural University 2015,34 (6) 140-145

植物遗传转化中农杆菌侵染与植物抗性反应相互作用的研究进展

作  者:
暴志茹;包满珠
单  位:
园艺植物生物学教育部重点实验室/华中农业大学园艺林学学院
关键词:
农杆菌介导的遗传转化;VIP1;防御反应;顽抗植物;农杆菌侵染;宿主细胞
摘  要:
根癌农杆菌介导的植物遗传转化是现代分子育种的重要手段之一,但农杆菌转化法具有外植体基因型依赖性,使得一些顽抗物种很难获得转化植株,有效利用参与转化过程的植物基因是进一步提高转化效率的可能路径。VIP1是一种bZIP转录因子,参与植物的免疫应答反应,但最近研究发现激活VIP1蛋白会促进农杆菌介导的转化过程,通过优化植物组织培养条件,增加植物体内VIP1的表达量可以提高遗传转化效率。总结植物与农杆菌之间相互作用的分子生物学过程,将为提高植物转化效率提供新的策略。
译  名:
Advances in Agrobacterium-mediated plant genetic transformation:the interaction between Agrobacterium infection and plant resistance response
作  者:
BAO Zhi-ru;BAO Man-zhu;Key Laboratory of Horticultural Plant Biology,Ministry of Education/College of Horticulture and Forestry Sciences,Huazhong Agricultural University;
关键词:
Agrobacterium-mediated transformation;;VIP1;;inner immunity reactions;;recalcitrant plants;;Agrobacterium infection;;host cell
摘  要:
Agrobacterium-mediated genetic transformation is one of the most important technologies for plant molecular breeding.However,the Agrobacterium-mediated genetic transformation system is plant genotype-dependent,and there are still a lot of recalcitrant plant species hard to be transformed.Taking advantage of plant genes that involved in the transformation steps might be a new pathway for improving transformation efficiency.VIP1,a bZIP transcription factor that is involved in the plant immunity responses.Recent studies demonstrated that Agrobacterium is able to activate and use VIP1 for transformation.Optimalizing the tissue culture condition to increase the expression of VIP1 can improve the transformation efficiency.Understanding the plant-Agrobacterium biological interaction processes could help developing new strategies for improving the transformation efficiency of the recalcitrant plant species.

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