关键词：
LiCl;β-catenin;毛囊;绒山羊
摘 要：
为研究不同剂量LiCl对绒山羊毛囊生长和β-连环蛋白(β-catenin)m RNA表达的影响,将分离的绒山羊初级毛囊随机分为5组,每组24个重复,对照组为William'E基础培养液。试验组在基础培养液中依次添加2,5,10和20mmol·L~(-1)的LiCl,培养期为7d,每日测量毛囊生长长度。运用荧光定量PCR技术测定各组β-cateninm RNA表达水平。结果表明:2~10 mmol·L~(-1)LiCl组的β-catenin m RNA表达水平和生长长度极显著高于对照组(p<0.01),毛囊形态维持良好;LiCl浓度为20mmol·L~(-1)组的β-catenin m RNA表达量及生长长度极显著低于对照组(p<0.01),体外培养毛囊生长受到抑制。添加2~10mmol·L~(-1)的LiCl可促进绒山羊毛囊生长,促使β-catenin m RNA表达水平提高,其中5mmol·L~(-1)为较优浓度。通过本试验研究发现,适量的LiCl在一定程度上有利于初级毛囊的生长及形态的维持,促进了β-catenin m RNA表达。
译 名：
Effect of LiCl on the Growth of Hair Follicles and the Expression of β-catenin in Cashmere Goat
作 者：
LIU Hai-jing;LIU Hai-ying;YANG Gui-qing;DONG Wei-gong;Animal Husbandry and Veterinary College, Shenyang Agricultural University;
关键词：
LiCl;;β-catenin;;hair follicle;;Cashmere goat
摘 要：
This experiment was to study in the hair follicle beta catenin(β-catenin) m RNA expression and the growth of hair follicle under the effect of different doses of LiCl. The cashmere goat primary follicles which have been separated were randomly divided into 5 groups. Each group has 24 replicates in this study. The control group of this study was William 'E basal medium.In the experimental group, LiCl was added in the order of 2, 5, 10 and 20 mmol·L~(-1), based on the basal medium. This training lasted for 7 days. The hair follicles' growth length was measured every day. Real-time PCR technology was used to determine the level of β-catenin m RNA expression in each group of hair follicle. The results showed that the expression level of β-catenin m RNA and the hair follicle's growth length were significantly higher(p<0.01) than the control group in the 2 to 10 mmol·L~(-1)LiCl groups. The shape of hair follicles maintained good in this group. The expression level of β-catenin m RNA and the hair follicle's growth length were significantly lower(p<0.01) than the control group in the 20 mmol·L~(-1)LiCl group. The hair follicle's growth length were inhibited in vitro. In conclusion, the adjunction of 2 to10 mmol·L~(-1)LiCl could promote hair follicle growth and the level of β-catenin m RNAs' expression, in which 5 mmol·L~(-1)has the best effect. Through this experimental study: a bit of LiCl could promote the growth of the primary follicles and maintain primary follicles of the form,promoted the expression ofβ-catenin m RNA.
