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当前位置: 首页 > 文章 > 激活Wnt/β-catenin信号通路抑制猪AMSCs向脂肪细胞分化 畜牧兽医学报 2010,41 (12) 1523-1528
Position: Home > Articles > Activation of Wnt/β-catenin Signaling Pathway Inhibits the Adipogenic Differentiation of Porcine Adipose-derived Mesenchymal Stem Cells Chinese Journal of Animal and Veterinary Sciences 2010,41 (12) 1523-1528

激活Wnt/β-catenin信号通路抑制猪AMSCs向脂肪细胞分化

作  者：
李惠侠;罗肖;刘荣鑫;杨映娟;杨公社
单  位：
南京农业大学动物科技学院;西北农林科技大学动物脂肪沉积与肌肉发育实验室;西北农林科技大学动物科技学院动物脂肪沉积与肌肉发育实验室南京农业大学动物科技学院;西北农林科技大学
关键词：
Wnt/β-catenin信号通路;LiCl;脂肪间充质干细胞;脂肪细胞;分化
摘  要：
本研究以猪脂肪间充质干细胞(AMSCs)为材料,通过LiCl对AMSCs细胞中Wnt/β-catenin信号通路第二信使β-联蛋白(β-catenin)的稳定作用,探讨体外激活Wnt/β-catenin信号通路对猪AMSCs向脂肪细胞分化的作用及其初步机制。应用细胞免疫化学染色鉴定AMSCs细胞表面分子CD44和CD105的表达;油红O染色法检测AMSCs成脂分化潜能;采用半定量RT-PCR及Westernblot等技术分析Wnt/β-catenin通路各因子及脂肪细胞分化转录因子的表达。结果显示,猪AMSCs表达CD44和CD105,并具有多向分化潜能;25mmol·L-1的LiCl处理AMSCs后,细胞中β-catenin蛋白表达明显增强;激活Wnt/β-catenin信号通路后,猪AMSCs在成脂诱导剂作用下向脂肪细胞分化的数目减少、细胞内甘油三酯含量降低,脂肪细胞分化转录因子C/EBPα和PPARγ的表达受到抑制。以上结果表明,激活Wnt/β-catenin信号通路能明显抑制猪AMSCs向脂肪细胞分化,其抑制作用可能是通过下调脂肪细胞分化转录因子PPARγ和C/EBPα的表达来实现的。
译  名：
Activation of Wnt/β-catenin Signaling Pathway Inhibits the Adipogenic Differentiation of Porcine Adipose-derived Mesenchymal Stem Cells
作  者：
LI Hui-xia1,2,LUO Xiao1,LIU Rong-xin1,YANG Ying-juan1,YANG Gong-she1*(1.Laboratory of Animal Fat Deposition and Muscle Development,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China;2.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)
关键词：
Wnt/β-catenin signaling pathway;LiCl;adipose-derived mesenchymal stem cells;adipocyte;differentiation
摘  要：
To investigate the effects and molecular mechanisms of Wnt/β-catenin signaling pathway in the adipogenic differentiation of adipose-derived mesenchymal stem cells(AMSCs),the porcine AMSCs was established,and the Wnt/β-catenin signaling pathway was activated by regulation of the pathway's second messenger β-catenin.The expression level of CD44 and CD105 of porcine AMSCs cells was evaluated by cellular immunochemistry technique.The adipogenic differentiation of AMSCs was analyzed by Oil red O staining.The expression of Wnt/β-catenin signaling pathway factors and the adipogenic transcription factors were checked by semi-quantitative RT-PCR and Western blot analysis.The results showed that the porcine AMSCs expressed of CD44 and CD105,and they have multiple differentiation potential.Wnt/β-catenin signaling pathway was activated by stabilization of cytoplasmic β-catenin with continuous treatment AMSCs by 25 mmol·L-1 LiCl.The number of adipocytes,and the content of triglyceride decreased during adipogenic induction differentiation of porcine AMSCs.In addition,the adipogenic transcription factors of CCAAT/enhancer-binding protein-α(C/EBPα) and peroxisome proliferator-activated receptor-γ(PPARγ) were repressed in the presence of LiCl.Taken together,the result indicated that activation of Wnt/β-catenin signaling pathway inhibits the adipogenic differentiation of porcine AMSCs,and its inhibition may due to reduce the expression of C/EBPα and PPARγ.

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