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Position: Home > Articles > Response Surface Methodology for Optimization of Medium Composition for Isomaltooligosaccharide Production by Aspergillus niger CU-1 FOOD SCIENCE 2012,33 (19) 216-222

响应面法优化黑曲霉发酵产低聚异麦芽糖培养基

作  者:
吴孔阳;王学军;周培华;黄时海;张云开;陈桂光;梁智群
单  位:
广西大学生命科学与技术学院;广西大学轻工与食品工程学院
关键词:
黑曲霉;α-葡萄糖苷酶;响应面分析法;培养基优化;低聚异麦芽糖
摘  要:
采用全因子试验、最陡爬坡试验以及Box-Behnken试验设计对黑曲霉CU-1(Aspergillus niger CU-1)发酵生产低聚异麦芽糖培养基的主要成分进行优化。结果表明:最优培养基成分为:麸皮浸汁体积分数4%、玉米浆添加量19.67g/L、NaNO3添加量2.24g/L、木薯淀粉糖化液添加量250g/L,在该培养条件下,在3.6L发酵罐中进行验证,发酵液中异麦芽糖、潘糖和异麦芽三糖总产量达到37.4%,低聚异麦芽糖总产量高达83.1%,说明Box-Behnken试验设计法用于黑曲霉发酵生产低聚异麦芽糖培养基优化是可行的,数学模型的预测值与实验观察值相符。
译  名:
Response Surface Methodology for Optimization of Medium Composition for Isomaltooligosaccharide Production by Aspergillus niger CU-1
作  者:
WU Kong-yang1,WANG Xue-jun2,ZHOU Pei-hua2,HUANG Shi-hai2,ZHANG Yun-kai2,CHEN Gui-guang2,LIANG Zhi-qun2,(1.Institute of Light Industry and Food Engineering,Guangxi University,Nanning 530004,China;2.College of Life Science and Technology,Guangxi University,Nanning 530005,China)
关键词:
Aspergillus niger;α-glucosidase;response surface methodology;medium optimization;isomaltooligosaccharides
摘  要:
The major fermentation medium components for isomaltooligosaccharide production by Aspergillus niger CU-1 were optimized by full factorial,steepest ascent and Box-Behnken designs.The optimal medium components were determined to be 4% wheat bran extract,19.67 g/L corn steep liquor,2.24 g/L NaNO3,and 250 g/L saccharified cassava starch.Fermentation of the optimized medium by Aspergillus niger CU-1 was carried out in a 3.6 L fermenter to obtain isomaltose,panose and isomaltotriose yield as high as 37.4%,an isomaltooligosaccharide yield as high as 83.1%.The good agreement between the predictive and actual values indicated that the mathematical model established based on BoxBehnken design was valid.

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