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Position: Home > Articles > Screening and Protoplast Mutagenesis of Aspergillus niger for Enhanced Endoinulinase Activity FOOD SCIENCE 2012,33 (9) 144-148

黑曲霉原生质体诱变选育内切型菊粉酶生产菌株

作  者:
李文利;曹泽虹;董玉玮;孙会刚;刘夕娟;唐盼
单  位:
徐州工程学院食品(生物)工程学院
关键词:
黑曲霉;菊粉酶;原生质体;诱变
摘  要:
从徐州市沛县河口镇秦庄村牛蒡种植基地腐烂牛蒡根附近采集土壤,经过初筛得到了3批菌株(包括从中国科学院购买菌株黑曲霉AS3.316),经过测定内切型菊粉酶酶活力(I)和外切型菊粉酶酶活力(S),筛选出I/S值大于10的菌株,共17株菌株。从17株黑曲霉菌株样本中,再筛选出一株产菊粉酶酶活力最高的菌株C122803,其酶活力为2.78U/mL。对菌株C122803进行原生质体制备及LiCl诱变后,得到一株内切型菊粉酶酶活力最高的菌株YY18,其酶活力为3.97U/mL,比出发菌株的酶活力提高了42.80%。
译  名:
Screening and Protoplast Mutagenesis of Aspergillus niger for Enhanced Endoinulinase Activity
作  者:
LI Wen-li,CAO Ze-hong,DONG Yu-wei,SUN Hui-gang,LIU Xi-juan,TANG Pan(College of Food and Bioengineering,Xuzhou Institute of Technology,Xuzhou 221008,China)
关键词:
Aspergillus niger;inulase;protoplast;mutagenesis
摘  要:
Soil was collected from the periphery of rotten burdock roots in the burdock planting base of Qinzhuang village,Hekou town,Pei county,Xuzhou municipality.Three batches of Aspergillus niger strains(including Aspergillus niger 3.316 strains) were isolated and 17 strains with an endoinulinase(I)-to-exoinulinase(S) activity ratio of higher than 10 were screened from them by secondary screening.Strain C122803 exhibited the highest I/S ratio(2.78 U/mL) among 17 screened strains.Strain YY18 with an endoinulinase activity of 3.97 U/mL was obtained from strain C122803 through protoplast preparation and LiCl mutagenesis.The activity obtained was 42.80% higher than that of the parent strain.

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