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Position: Home > Articles > Optimization of Immobilization of Aspergillus niger Cells with Fructosyltransferase Activity FOOD SCIENCE 2013,34 (21) 172-176

产果糖基转移酶的黑曲霉细胞固定化条件优化

作  者:
张颖;姚盟成;荆玮;路福平;李玉
单  位:
中国环境管理干部学院生态学系;天津科技大学生物工程学院
关键词:
低聚果糖;黑曲霉;果糖基转移酶;固定化细胞;响应面试验
摘  要:
以卡拉胶为固定化载体,对含有果糖基转移酶的黑曲霉细胞进行固定化。通过单因素和Box-Behnken试验,得到其最优固定化工艺为:卡拉胶9.4g/L、多乙烯多胺9g/L、戊二醛10g/L、KCl 15g/L、菌浓100g/L,在此最优工艺条件下低聚果糖含量达到最大值40.12%,与模型预测值接近。优化后的工艺可为低聚果糖的生产提供技术参考。
译  名:
Optimization of Immobilization of Aspergillus niger Cells with Fructosyltransferase Activity
作  者:
ZHANG Ying;YAO Meng-cheng;JING Wei;LU Fu-ping;LI Yu;Department of Ecology,Environmental Management College of China;College of Biotechnology,Tianjin University of Science and Technology;
关键词:
fructooligosaccharide;;Aspergillus niger;;fructosyltransferase;;immobilized cells;;response surface methodology
摘  要:
Aspergillus niger cells with fructosyltransferase were immobilized by entrapping into carrageenan.Five immobilization parameters were primarily studied by one-factor-at-a-time method,and the optimization of three of them(concentration of carrageenan,glutaraldehyde and polyethylene polyamine,PEPA) was explored by using a Box-Behnken design coupled with response surface methodology.The results showed the optimal process conditions were 9.4 g/L carrageenan,10 g/L glutaraldehyde,15 g/L KCl,9 g/L PEPA and 100 g/L Aspergillus niger cells.Under the optimal conditions,the maximum yield of fructooligosaccharides(FOS) was 40.12%,which was consistent with the predicted value,and the established model was reliable in prediction.

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