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当前位置: 首页 > 文章 > 血管内皮生长因子对绵羊卵母细胞体外受精及胚胎发育的影响 甘肃农业大学学报 2008,43 (4) 33-37
Position: Home > Articles > Effects of VEGF on the ovine oocytes maturation and the early embryo development in vitro Journal of Gansu Agricultural University 2008,43 (4) 33-37

血管内皮生长因子对绵羊卵母细胞体外受精及胚胎发育的影响

作  者：
曹忻;罗海玲;石国庆;赵有璋
单  位：
甘肃农业大学动物科技学院;中国农业大学动物科技学院;新疆农垦科学院畜牧所
关键词：
血管内皮生长因子;体外成熟;体外受精;体外发育;绵羊
摘  要：
为了研究血管内皮生长因子(vascular endothelial growth factor,VEGF)对绵羊卵母细胞体外成熟和成熟后卵母细胞体外受精卵的早期发育的影响,在细胞培养过程中分别在体外成熟培养液、体外受精液及体外胚胎发育液中添加5、10 ng/mL VEGF.结果显示,添加VEGF组卵母细胞的体外成熟率由对照组的80.68%提高到了86.09%和85.22%(P<0.01),成熟卵母细胞的卵裂率由69.38%提高到了75.18%和73.48%(P>0.05),5 ng/mL添加组的桑葚胚率(45.45%)和囊胚率(30.06%)极显著(P<0.01)高于对照组(37.61%;23.65%);而10 ng/mL添加组的桑葚胚率(40.25%)和囊胚率(27.80%)低于5 ng/mL添加组的,高于对照组,但差异不显著.据此认为5 ng/mL VEGF的添加量比10 ng/mL VEGF添加量能更有效的促进绵羊卵母细胞体外成熟、体外受精和胚胎早期发育.
译  名：
Effects of VEGF on the ovine oocytes maturation and the early embryo development in vitro
作  者：
CAO Xin1,2,LUO Hai-ling2,SHI Guo-qing3,ZHAO You-zhang1(1.College of Animal Science & Technology,Gansu Agricultural University,Lanzhou 730070,China;2.College of Animal Science & Technology,China Agricultural University,Beijing 100094,China;3.Xinjiang Academy of Agriculture and Reclamation Science,Shihezi 832000,China)
关键词：
vascular endothelial growth factor(VEGF);IVM;IVF;IVC;ovine
摘  要：
To investigate the effect of vascular endothelial growth factor(VEGF) on the ovine oocytes maturation and the early embryo development in vitro,5 ng/mL and 10 ng/mL VEGF were supplemented to the maturation medium,the fertilization medium and the culture medium respectively.The results showed that the maturation rate was increased significantly(P<0.01)from 80.68 % in the control(0 ng/mL VEGF) to 86.09 % and 85.22 % in the treatment groupⅠ(5 ng/mL VEGF)and the treatment groupⅡ(10 ng/ml VEGF)respectively.The cleavage rate was increased from 69.38 % in the control group to 75.18 % and 73.48 %(P>0.05) in the treatment groupⅠand the treatment groupⅡ respectively.The development rates of morulae and blastocyst in the treatment groupⅠ(45.45 % and 30.06 %) were significantly higher(P<0.01)than that of the control group(37.61 % and 23.65 %),and the development rates of morulae and blastocyst in the treatment groupⅡ(40.25 % and 27.80 %)were lower than that of the treatment groupⅠ(P>0.05)and higher than that of the control group(P>0.05).These results indicated that maturation medium,fertilization medium and the culture medium with 5 ng/ml of recombinant human VEGF165 significantly improved the maturation and fertilization of ovine oocytes and consequently the rate of embryos development were markedly enhanced.

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