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当前位置: 首页 > 文章 > 放射配体受体分析法对日本血吸虫体内5-HT受体的测定 动物医学进展 2003,24 (1) 97-99
Position: Home > Articles > The Detection of 5-HT Receptor by RLBA in Schistosoma japonicum Progress in Veterinary Medicine 2003,24 (1) 97-99

放射配体受体分析法对日本血吸虫体内5-HT受体的测定

作  者：
王丽平;沈丽琳;陈伟华
单  位：
南京农业大学动物医学院
关键词：
日本血吸虫;RLBA;5-羟色胺受体;螺环哌啶酮;Bmax;Kd
摘  要：
采用放射配体-受体分析法(RLBA)测定了日本血吸虫体内5-羟色胺(5-HT)受体及螺环哌啶酮(spiroperidol)对5-HT受体与3H-5-HT结合反应的影响。结果证实:日本血吸虫体内存在5-HT受体,与配体5-HT可进行饱和的、特异的结合,其Scatchard图呈下凹曲线,表明5-HT受体的复杂性和不均一性。其最大结合容量(Bmax)为210.4fmol/mgprotein,解离常数(Kd)为0.585pmol/L,螺环哌啶酮对3H-5-HT与受体的结合无影响,计算Bmax为270.3fmol/mgprotein,Kd为0.327pmol/L,与前值比较,差异不显著(P>0.05)。
译  名：
The Detection of 5-HT Receptor by RLBA in Schistosoma japonicum
作  者：
WANG Liping,SHEN Lilin,CHEN Weihua(Department of Veterinary Medicine, Nanjing Agric Univ Nanjing,jiangsu,210095,China)
关键词：
Schistosoma japonicum; RLBA;5HT receptor;Bmax;spiroperidol
摘  要：
5HT receptor in Schistosoma japonicum in vivo was determined by the radioligand binding assay(RLBA).The result showed that there were 5HT receptors in Schistosoma japonicum and the maximal association constant(Bmax)and Kd of 5HT receptor on Schistosoma japonicum were 210.4 fmol/mg protein and 0.585 pmol/L, respectively. The curve of Scatchard plot was of pit. It indicated that there was a greater association content and a higher affinity between the ligand and its 5HT receptor, but the binding was inhomogeneous and complicated.In addition, the result showed that spiroperidol had no effect on the binding between 5HT and it's receptor. The Bmax and Kd values were 270.3 fmol/mg protein and 0.327 pmol/L, respectively. The values were not significant compared with the above (P>0.05). The result suggested that the site binding with spiroperidol was not the same with 5HT. 

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