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当前位置: 首页 > 文章 > 低温下不同H_2O_2抑制剂对四季秋海棠花色素苷合成的影响 河南农业科学 2018 (11) 110-115
Position: Home > Articles > Effects of Different H_2O_2 Inhibitors on Anthocyanin Biosynthesis Induced by Low Temperature in Beginia semperflorens Journal of Henan Agricultural Sciences 2018 (11) 110-115

低温下不同H_2O_2抑制剂对四季秋海棠花色素苷合成的影响

作  者：
王阳;吴亚蓓;齐睿;田耕;白雪;李永华;张开明
单  位：
河南省农业科学院农业经济与信息研究所;河南农业大学林学院
关键词：
四季秋海棠;花色素苷;低温;H2O2;诱导机理
摘  要：
为了确定不同H_2O_2抑制剂对低温诱导植物花色素苷合成的影响,探讨此过程中不同来源H_2O_2的作用途径和机制,以四季秋海棠为研究试材,采用酶活性分析和qRT-PCR等方法,测定了不同处理下SOD、POD、PAO、GO酶的活性和H_2O_2、MDA的含量,以及花色素苷合成相关酶基因的表达水平。结果表明:低温显著增加了四季秋海棠叶片的SOD、POD、PAO、GO酶活性,促进了H_2O_2的积累和MDA含量的升高,最终引起花色素苷合成相关酶基因(BsPAL、BsCHS、BsF3H、BsANS)表达上调和花色素苷含量的增加;施用光合电子传递链的抑制剂DCMU、SOD的抑制剂DDC、GO酶的抑制剂OX和NADPH氧化酶的抑制剂DPI预处理6 h再给予低温处理,均不同程度地降低了SOD、POD、PAO、GO的活性和H_2O_2、MDA的含量,花色素苷合成相关酶基因表达水平下降,导致花色素苷合成受阻。因此,不同抑制剂预处理,分别降低由光合电子传递链、SOD、GO、NADPH氧化酶介导产生的H_2O_2后,均不同程度地减少了花色素苷含量,表明光合电子传递链、SOD、GO和NADPH氧化酶介导产生的H_2O_2可能参与了低温诱导四季秋海棠叶片花色素苷合成的过程。
译  名：
Effects of Different H_2O_2 Inhibitors on Anthocyanin Biosynthesis Induced by Low Temperature in Beginia semperflorens
作  者：
WANG Yang;WU Yabei;QI Rui;TIAN Geng;BAI Xue;LI Yonghua;ZHANG Kaiming;College of Forestry,Henan Agricultural University;Agricultural Economy and Information Research Institute,Henan Academy of Agricultural Sciences;
单  位：
WANG Yang%WU Yabei%QI Rui%TIAN Geng%BAI Xue%LI Yonghua%ZHANG Kaiming%College of Forestry,Henan Agricultural University%Agricultural Economy and Information Research Institute,Henan Academy of Agricultural Sciences
关键词：
Beginia semperflorens;;Anthocyanin;;Low temperature;;H2O2;;Induction mechanism
摘  要：
To analyze the effects of different H_2O_2 inhibitors on anthocyanin biosynthesis induced by low temperature,to discuss the function principle and function way of different originations of H_2O_2 produced in this process,the activity of SOD,POD,PAO and GO enzymes,the content of H_2O_2 and MDA,and the transcription levels of anthocyanin biosynthesis genes were measured under different treatments in Begonia semperflorens,using the methods of enzyme-activity analysis and qRT-PCR,etc..The results showed that:The activities of SOD,POD,PAO and GO were significantly increased by low temperature,causing H_2O_2 accumulation and an increase in MDA content in Beginia semperflorens.The transcription levels of anthocyanin biosynthesis genes(namely BsPAL,BsCHS,BsF3 H and BsANS) and anthocyanin content were consequently soared in Beginia semperflorens;After the pretreatment with DCMU(an inhibitor of photosynthetic electron transport),DDC(an inhibitor of SOD),OX(an inhibitor of GO) and DPI(an inhibitor ofNADPH oxidase) for 6 hours and then treated with low temperature,in different degrees,the activities of SOD,POD,PAO and GO all decreased,the contents of H_2O_2 and MDA decreased either;blocked partially anthocyanin biosynthesis by decreasing the transcription levels of anthocyanin biosynthesis genes.As a result,pretreatments of all the test inhibitors decreased the contents of H_2O_2 came from photosynthetic electron transport,SOD,GO and NADPH oxidase;and anthocyanin content was also decreased to different level in Beginia semperflorens.We therefore suggested that H_2O_2 produced by photosynthetic electron transport,SOD,GO and NADPH oxidase may be involved in low temperature induced anthocyanin biosynthesis in Beginia semperflorens.

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