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Position: Home > Articles > Application of Microwave Technology in Protein Extraction from Swim Bladder of Silver Carp FOOD SCIENCE 2009,30 (16) 149-152

微波技术在鱼鳔蛋白提取中的应用

作  者:
段振华;孙小苓;汪菊兰
单  位:
海南大学;海南大学食品学院
关键词:
鱼鳔;微波技术;提取率;蛋白质;考马斯亮蓝
摘  要:
选取新鲜鱼鳔为原料,以蛋白质提取率为指标,采用考马斯亮蓝法测定提取液中的蛋白质含量,分别研究固液比、微波萃取时间和微波功率等因素对鱼鳔蛋白提取的影响,并通过正交试验优化微波提取鱼鳔蛋白的工艺,得出在固液比1:30、萃取时间2.5min、萃取功率300W条件下,鱼鳔蛋白质的提取率最高达22.79%。
译  名:
Application of Microwave Technology in Protein Extraction from Swim Bladder of Silver Carp
作  者:
DUAN Zhen-hua1,2,SUN Xiao-ling1,WANG Ju-lan2(1.College of Food Science and Technology,Hainan University,Haikou 570228,China;2.Key Laboratory of Tropic Biological Resources of Ministry of Education,Hainan University,Haikou 570228,China)
关键词:
swim bladder;microwave technology;extraction yield;protein;Coomassie brilliant blue
摘  要:
The microwave extraction of protein from swim bladders of silver carps was investigated.Four crucial extraction parameters were optimized to attain the highest extraction yield of protein by single-factor and orthogonal array designs.The optimal conditions for attain the maximum extraction yield of 22.79% were determined as follows:with the ratio of material to liquid(water) of 1:30,extraction at 300 W for 2.5 min.This study will provide a practical reference for the application of microwave extraction in the preparation of protein from swim bladders.

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