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Position: Home > Articles > Study on Screening, Identification and Fermentation Conditions of Antagonistic Fungi against Agrobacterium tumefacie FOOD SCIENCE 2007,28 (2) 166-168

根癌农杆菌拮抗真菌的筛选、鉴定及发酵条件研究

作  者:
李祝;宋宝安;郁建平;肖洋;李颖;梁宗琦
单  位:
贵州省产品质量检验检测院;贵州大学生命科学学院;贵州大学精细化工研究中心
关键词:
根癌农杆菌;黑曲霉;筛选;发酵
摘  要:
从多种植物根际土壤中分离到52株真菌菌株,通过琼脂柱法初筛及摇瓶复筛,筛选出1株对根癌农杆菌(Agrobacterium tumefaciens T-37)有明显抑制效果的菌株xj,经分类鉴定确定为黑曲霉(Aspergillus niger),并对之发酵条件进行了研究,结果表明:以2%果糖、3%牛肉膏为液体发酵培养基,10%装液量,起始pH6.0,25℃,140r/min,发酵5d,抑菌圈直径可达到35.28±0.32mm。
译  名:
Study on Screening, Identification and Fermentation Conditions of Antagonistic Fungi against Agrobacterium tumefacie
作  者:
LI Zhu1,SONG Bao-an1,YU Jian-ping2,XIAO Yang3,LI Ying2,LIANG Zong-qi2 (1.Research and Development Center of Fine Chemicals, Guizhou University, Guiyang 550025, China; 2.College of Life Science, Guizhou University, Guiyang 550025, China; 3.Institute of Products Detection and Determination of Guizhou Province, Guiyang 550003, China)
关键词:
Agrobacterium tumefaciens;Aspergilluse niger;screen;fermentation
摘  要:
52 fungi strains were isolated from different rhizospheric soil samples, among which a strain xj to inhibit the growth of Agrobacterium tumefaciens T-37 was identified as Aspergilluse niger after being screened by agar column method and selected by liquid fermentation. The fermentation conditions for the Aspergilluse niger xj strain were optimized as follows: 2 % fructose, 3% beef extract as liquid ferment medium, 10% volume of substrate, initial pH6.0, 25℃,and 140r/min, for 5 days, while the inhibiting zones diameter was up to 35.28±0.32mm.

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