当前位置: 首页 > 文章 > 毕赤酵母表达外源蛋白糖基化研究进展 福建畜牧兽医 2014 (2) 20-22
Position: Home > Articles > Glycosylation of heterologous proteins in Pichia pastoris Fujian Journal of Animal Husbandry and Veterinary Medicine 2014 (2) 20-22

毕赤酵母表达外源蛋白糖基化研究进展

作  者:
杨婕
单  位:
福建师范大学生命科学学院
关键词:
毕赤酵母;O-糖基化;N-糖基化;糖基人源化
摘  要:
毕赤酵母作为表达外源蛋白的宿主,不仅具有原核生物表达系统的优点,如培养成本低、遗传操作简单、表达效率高等,还可以对表达的外源蛋白进行翻译后修饰加工,如蛋白质折叠、二硫键形成和糖基化等,因而有其独特的优势。在分泌蛋白修饰中,糖基化是一种重要修饰方式,影响蛋白的结构与功能。本文就毕赤酵母对表达的分泌蛋白进行糖基化修饰及糖基人源化改造研究进展进行综述。
译  名:
Glycosylation of heterologous proteins in Pichia pastoris
作  者:
Yang Jie;College of Life Sciences,Fujian Normal University;
关键词:
Pichia pastoris O-glycosylation N-glycosylation humanized glycosylation
摘  要:
Pichia pastoris as the host for the expression of recombinant proteins, has not only the advantages of prokaryotic expression system such as inexpensive in culture, ease of genetical manipulation, high levels of protein expression, but also has post-translational protein processing capabilities like other higher eukaryotes such as protein folding, formation of disulfide bond and glycosylation. Glycosylation is an important form of posttranslational modification in secreted proteins and affects the structure and function of these proteins. In this review, we discuss protein glycosylation and humanized glycosylation engineering in Pichia pastoris.

相似文章

计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊