当前位置: 首页 > 文章 > 木聚糖酶体外酶解小麦麸皮相关技术参数研究 安徽农业大学学报 2011,38 (2) 248-251
Position: Home > Articles > Optimization of technical parameters of wheat bran hydrolysis with xylanase in vitro Journal of Anhui Agricultural University 2011,38 (2) 248-251

木聚糖酶体外酶解小麦麸皮相关技术参数研究

作  者:
黄世霞;王在贵;刘朝良;刘婧;吴东;张宏福
单  位:
安徽省农业科学院畜牧兽医研究所;中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;安徽农业大学生命科学学院
关键词:
木聚糖酶;麸皮;酶解
摘  要:
木聚糖酶可以降解饲料中木聚糖,消除其抗营养作用,提高饲料消化利用率。本试验采用还原糖法研究了不同酶解时间、温度、pH值及不同酶浓度条件对木聚糖酶酶解小麦麸皮效果的影响,并在模拟动物胃环境条件下研究酶解麸皮的效果。结果表明,木聚糖酶对麸皮酶解的最适温度为50℃,最适pH为5.5;最适酶添加浓度在0.02 I U.mL-1,最适反应时间为20 min,经胃蛋白酶处理4 h后还原糖释放量下降不大,处理时间对酶活的影响不明显。
译  名:
Optimization of technical parameters of wheat bran hydrolysis with xylanase in vitro
作  者:
HUANG Shi-xia1,WANG Zai-gui 1,LIU Chao-liang,LIU Jing1,WU Dong2,ZHANG Hong-fu3 (1.School of Life Science,Anhui Agricultural University,Hefei 230036; 2.Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Sciences,Hefei 230031; 3.State Key Laboratory of Animal Nutrition,Institute of Animal Science,Chinese Academy of Agricultural Sciences Beijing 100193)
关键词:
xylanase;wheat bran;hydrolysis
摘  要:
Xylanase could eliminate or reduce xylan of feeds which was anti-nutrition to the animal,and en-hanced feed digestibility.Reducing sugar method was used to study the influences of the enzyme on wheat bran,and some important indices were measured.The results showed that the optimal condition for wheat bran enzy-molysis was:50℃,pH5.5,0.02 I U·mL-1 enzyme concentration,reaction for 20 min.Then the pig stomach en-vironment in vitro was simulated,and the result suggested that the treatment of pepsin did not reduce the release of the reducing sugar remarkably.Moreover,the activity of the xylanase in this condition was stable.

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