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当前位置: 首页 > 文章 > 鸭瘟病毒的分离鉴定及其UL2、TK基因序列分析 福建农业学报 2016,31 (12) 1257-1261
Position: Home > Articles > Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes Fujian Journal of Agricultural Sciences 2016,31 (12) 1257-1261

鸭瘟病毒的分离鉴定及其UL2、TK基因序列分析

作  者：
刘荣昌;黄瑜;卢荣辉;傅秋玲;江斌
关键词：
鸭瘟病毒;分离鉴定;UL2基因;TK基因;序列分析
摘  要：
2015年,福建省福州地区2个鸭场(M18肉鸭场及蛋鸭场)发生大量鸭死亡,初诊疑似鸭瘟。为明确其病原,分别采集病死鸭肝脏、食道样品进行鸭瘟病毒特异性PCR检测和病毒分离,对获得的2株病毒分离株分别进行鸭已知病原的检测,确定均为鸭瘟病毒。对新分离鉴定的2株鸭瘟病毒株的UL2基因进行序列测定及分析发现,均具有强毒株的分子特征;经TK基因序列测定及分析表明,2株新分离的鸭瘟病毒株与强毒代表株、弱毒疫苗株的TK基因核苷酸同源性均高达98.7%。
译  名：
Isolation and Identification of Duck Plague Virus and Sequence Analysis on Its UL2 and TK Genes
作  者：
LIU Rong-chang;HUANG Yu;LU Rong-hui;FU Qiu-ling;JIANG Bin;WAN Chun-he;FU Guang-hua;CHENG Long-fei;SHI Shao-hua;CHEN Hong-mei;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fujian Animal Diseases Control Technology Center,Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention;College of Animal Science and Technology,Fujian Agriculture and Forestry University;
关键词：
virulent duck plague virus;;isolation and identification;;UL2 gene;;TK gene;;sequence analysis
摘  要：
In March 2015,massive deaths happened to the M18 Duck Farm and an egg-laying duck farm in Fuzhou.It was suspected of an outbreak of duck plaque.Samples of liver and esophagus from the dead birds were collected for pathogenic identification using PCR and virus isolation.Two strains with virulent characteristics were thus identified.Subsequently,a sequence analysis on their UL2 genes further confirmed the classification.In addition,the TK gene sequences of the two isolated virus strains showed a nucleotide homology of 98.7% with those of the common virulent strain as well as the vaccine strain.

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