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Position: Home > Articles > Extraction Genomic DNA from Endophytic Actinomyces Using Benzyl Chloride Northern Horticulture 2010 (13) 124-126

氯化苄法提取植物内生放线菌基因组DNA

作  者:
刘建利
单  位:
北方民族大学生物科学与工程学院
关键词:
氯化苄;植物内生放线菌;基因组DNA
摘  要:
以植物内生放线菌菌株GL0806123为试材,用氯化苄快速提取植物内生放线菌菌株GL0806123基因组DNA。建立用氯化苄提取植物内生放线菌基因组DNA的方法。结果表明:用氯化苄法能够成功提取植物内生放线菌菌株GL0806123基因组DNA,以此为模板PCR扩增出16S rDNA区段,测序后可用于菌种鉴定。氯化苄法是一种快速提取植物内生放线菌基因组DNA的方法。
译  名:
Extraction Genomic DNA from Endophytic Actinomyces Using Benzyl Chloride
作  者:
LIU Jian-li (College of Biological Sciences and Engineering,North University for Ethnics,Yinchuan,Ningxia 750021)
关键词:
benzyl chloride;endophytic actinomyces;genomic DNA
摘  要:
Taking endophytic actinomyces GL0806123 as materials,the extraction genomic DNA using benzyl chloride were investigated in this paper.The study aimed to establish a novel method to extract the genomic DNA from endophytic actinomyces using benzyl chloride.The results showed that the genomic DNA extraction was had extracted successfully from endophytic actinomyces using benzyl chloride.And 16S rDNA was amplification with this DNA as PCR templates,then GL0806123 was identified based on this 16S rDNA sequence.Benzyl chloride method was a rapid method to extract the genomic DNA from endophytic actinomyces.

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