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Position: Home > Articles > Process Optimization for Pigment Extraction from Chestnut Shell with Cellulase FOOD SCIENCE 2012,33 (2) 149-153

纤维素酶法提取板栗壳色素的工艺条件优化

作  者:
李孚杰;潘丹贞;张丛兰;周露;杨芳
单  位:
湖北大学知行学院生物工程系
关键词:
板栗壳;色素;提取;响应面分析法
摘  要:
以板栗壳为原料,通过单因素试验,考察提取剂乙醇体积分数、提取时间、提取温度、纤维素酶添加量、料液比和提取液pH值对板栗壳色素提取效果的影响。在单因素试验的基础上,根据中心组合(Box-Behnken)试验设计原理,采用四因素三水平的响应面分析法,确定提取板栗壳色素的最佳工艺条件为pH6、纤维素酶添加量0.6%、提取温度94℃、提取时间120min。
译  名:
Process Optimization for Pigment Extraction from Chestnut Shell with Cellulase
作  者:
LI Fu-jie,PAN Dan-zhen,ZHANG Cong-lan,ZHOU Lu,YANG Fang(Department of Bioengineering,Zhixing College of Hubei University,Wuhan 430011,China)
关键词:
chestnut shells;pigment;extraction;response surface methodology
摘  要:
In this study,cellulase was used to extract pigment from chestnut shells.The effects of ethanol concentration,extraction time,extraction temperature,cellulase dosage,material/liquid ratio and pH on extraction rate of pigments were investigated.Based on one-factor-at-a-time experiments,Box-Behnken central composite design was used to optimize the extraction conditions.The results showed that the optimal extraction conditions were pH of 6,cellulase dosage of 0.6%,extraction temperature of 94 ℃,and extraction time of 120 min.

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