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Position: Home > Articles > Continuous Lactose Hydrolysis in Milk with Osmotic K.lactis Cells FOOD SCIENCE 2007,28 (5) 254-257

渗透性K.lactis细胞内乳糖酶水解牛乳中乳糖的研究

作  者:
李素芬;陈占洲;刘建福;陈庆森
单  位:
河北工程大学农学院;河北工程大学农学院,河北工程大学农学院,天津食品生物技术重点实验室 天津商学院生物工程系,天津食品生物技术重点实验室,天津商学院生物工程系,,河北邯郸056001,河北邯郸056001,天津300134,天津300134
关键词:
填充床反应器;渗透性K.lactis细胞;乳糖酶;连续水解
摘  要:
应用填充床细胞反应器,以渗透性K.lactis细胞内乳糖酶连续催化牛乳中乳糖的水解。渗透性K.lactis细胞内乳糖酶的热失活遵循一级反应动力学,40℃时酶的半衰期为9.5h,表观米氏常数为0.39mmol/ml,最大反应速率V_(max)为0.188mmol/min。在2.6cm×50cm填充床生物反应器上,渗透性K.lactis细胞内乳糖酶催化牛乳中乳糖水解的适宜体积流速为1.0ml/min,酶反应的适宜温度40℃,反应速率达到动态平衡后生成的葡萄糖的质量浓度为45.2mg/ml,牛乳中乳糖的水解率为91.3%。
译  名:
Continuous Lactose Hydrolysis in Milk with Osmotic K.lactis Cells
作  者:
LI Su-fen~1 CHEN Zhan-zhou~1 LIU Jian-fu~2 CHEN Qing-sen~2 (1.School of Agriculture,Hebei Engineering University,Handan 056001,China; 2.Tianjin Key Laboratory of Food Biotechnology,Department of Bioengineering,Tianjin University of Commerce,Tianjin 300134,China)
关键词:
packed-bed reactor;;osmotic K.lactis cell;;lactase;;lactose;;continuous hydrolysis
摘  要:
Osmotic K.lactis cells were packed into packed bed reactor to hydrolyze lactose in milk.The thermal inactivation of lactase entrapped in K.lactis cells followed the first-order kinetics,and its half-life was 9.5h at 40℃.Appearent K_m constant and maximum reaction rate for the enzyme were 0.39 mmol/ml and 0.188 mmol/min,respectively.The optimal reaction parameters for hydrolysis of lactose in milk with osmotic K.lactis cells packed in 2.6 cm×50 cm column are:flow rate 1.0 ml/min at 40℃.Under these conditions,hydrolyzed glucose concentration reaches 45.2 mg/ml.

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