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当前位置: 首页 > 文章 > 多孔陶瓷球固定化β-葡萄糖苷酶的研究 南京林业大学学报(自然科学版) 2013,37 (3) 110-116
Position: Home > Articles > Immobilization of β-glucosidase on porous ceramic balls Journal of Nanjing Forestry University(Natural Sciences Edition) 2013,37 (3) 110-116

多孔陶瓷球固定化β-葡萄糖苷酶的研究

作  者：
韦策;陆青山;勇强;余世袁
单  位：
南京林业大学化学工程学院
关键词：
β-葡萄糖苷酶;固定化;多孔陶瓷球;填充床反应器
摘  要：
以多孔陶瓷球为载体固定化β-葡萄糖苷酶,对固定化条件进行优化,确定陶瓷球固载β-葡萄糖苷酶的条件为:5 g陶瓷球载体与3 mL酶液,在4℃摇床中振荡交联反应2 h,低温干燥后,用含有0.3 g油性胶黏剂的溶液对固定化酶进行包封,酶液中β-葡萄糖苷酶浓度为36.5μmol/(mL.min),缓冲液pH为4.8,戊二醛体积分数0.5%。陶瓷球固定化β-葡萄糖苷酶的最适反应pH为4.8,游离酶pH为4.2,固定化和游离酶的最适反应温度均为60℃;固定化酶的酸碱、热和储存稳定性优于游离酶;固定化和游离酶的反应动力学常数Km分别为7.9 mmol/L和0.73 mmol/L;利用固定化酶重复分批酶解纤维二糖-葡萄糖混合糖液,前5批的酶解得率在80%以上。
译  名：
Immobilization of β-glucosidase on porous ceramic balls
作  者：
WEI Ce1,2,LU Qingshan1,YONG Qiang1,YU Shiyuan1(1.Key Laboratory of Forest Genetics and Biotechnology,College of Chemical Engineering,Nanjing Forestry University, Nanjing 210037,China;2.College of Biotechnology and Pharmaceutical Engineering,Nanjing University of Technology,Nanjing 211816,China)
关键词：
β-glucosidase;immobilization;porous ceramic ball;packed-bed reactor
摘  要：
With the objective of achieving practicable β-glucosidase immobilization,porous ceramic balls were taken as the carriers,and the conditions on β-glucosidase immobilization were studied.The optimum operation of β-glucosidase immobilization was as follows:5 g porous ceramic balls were put into 0.3 mL β-glucosidase solution(pH 4.8),in which the concentrations of β-glucosidase and glutaraldehyde were 36.5 μmol/(mL·min) and 0.5%,respectively.The mixture was shaken at 4 ℃ for 2 h and then dried under low temperature.The immobilized β-glucosidase was obtained after enveloped by a solution which contained 0.3 g oil-based adhesive.The optimum reaction pH of immobilized β-glucosidase was 4.8,whereas the reaction pH of free β-glucosidase was 4.2.The optimum reaction temperatures of immobilized and free β-glucosidase were 60 ℃.Immobilized β-glucosidase offered the better properties than free β-glucosidase in pH,thermal and storage tests.The Km of immobilized and free β-glucosidase were 7.9 mmol/L and 0.73 mmol/L,respectively.A sugar mixture containing cellobiose and glucose was enzymatically hydrolyzed by the immobilized β-glucosidase.The yields of cellobiose conversion could be higher than 80% in 5 repeated batch operations.

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