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当前位置: 首页 > 文章 > 高效液相色谱-蒸发光散射法测定酶法合成低聚乳果糖的含量 食品科学 2010,31 (16) 180-183
Position: Home > Articles > High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose FOOD SCIENCE 2010,31 (16) 180-183

高效液相色谱-蒸发光散射法测定酶法合成低聚乳果糖的含量

作  者：
代志凯;廖春龙;印遇龙;阮征
单  位：
南昌大学
关键词：
高效液相色谱;蒸发光散射检测器;低聚乳果糖
摘  要：
目的:建立简便、准确、灵敏地测定酶法合成中低聚乳果糖含量的液相色谱方法。方法:酶法合成低聚乳果糖的溶液经离心后稀释,使用氨基柱分离,乙腈-水(体积比75:25)为流动相,流速1.0mL/min,蒸发光散射检测器漂移管温度70℃,载气为空气,压力0.35MPa。结果:低聚乳果糖的线性范围为0.1281～4.1000mg/mL(R2=0.9994),最低检出限为0.01mg/mL,平均加标回收率为100.28%,RSD为3.07%。结论:该方法操作简便、快速、准确,用于酶法合成低聚乳果糖的测定,结果令人满意。
译  名：
High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose
作  者：
DAI Zhi-kai1,2,LIAO Chun-long1,2,YIN Yu-long1,2,3,RUAN Zheng1,2,* (1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ; 2.College of Life Science and Food Engineering, Nanchang University, Nanchang 330031, China; 3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China)
关键词：
high-performance liquid chromatography;evaporative light scattering detection;lactosucrose
摘  要：
Objective: To develop a simple, accurate and sensitive high-performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) for the determination of enzymatically prepared lactosucrose (LS) in our laboratory. Methods: The reaction product containing LS was centrifugated and the supernatant was diluted and separated on a Kromasil NH2 column using acetonitrile/water (75:25, V/V) mixture as mobile phase. The ELSD drift tube temperature was set at 70 ℃ and the air pressure at 0.35 MPa. Results: The linear range of LS was between 0.1281 mg/mL and 4.1000 mg/mL (R2 = 0.9994). The method detection limit was 0.01 mg/mL. The average spike recovery at 5 levels was 100.28%, with a relative standard deviation (RSD) of 3.07%. Conclusion: This method is simple, fast and accurate, and can be applied to the determination of LS.

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