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Position: Home > Articles > Susceptibility of rice lepidopterans to four insecticidal proteins from Bacillus thuringiensis Plant Protection 2007,33 (6) 78-80

水稻螟虫对4种Bt杀虫蛋白的敏感性测定

作  者:
张广林;韩成香;姚洪渭;叶恭银
单  位:
浙江大学昆虫科学研究所
关键词:
苏云金杆菌;Cry蛋白;二化螟;大螟;稻纵卷叶螟
摘  要:
采用人工饲料喂毒法和浸叶喂毒法测定了二化螟、大螟和稻纵卷叶螟初孵幼虫对4种Bt杀虫蛋白的敏感性。二化螟对Cry1Ab、Cry1Ac、Cry1C和Cry2A的LC50分别为1.739、2.6452、.794 ng/mg和15.965 ng/mg;大螟依次为2.455、10.046、11.803 ng/mg和35.359 ng/mg;稻纵卷叶螟依次为4.000、1.611、69.031μg/mL和12.708μg/mL。作者就不同水稻螟虫对不同Cry蛋白的敏感性差异及其在转基因抗虫作物中的应用作了讨论。
译  名:
Susceptibility of rice lepidopterans to four insecticidal proteins from Bacillus thuringiensis
作  者:
Zhang Guanglin,Han Chengxiang,Yao Hongwei,Ye Gongyin(Institute of Insect Science,Zhejiang University,Hangzhou 310029,China)
关键词:
Bacillus thuringiensis;Cry protein;Chilo suppressalis;Sesamia inferens;Cnaphalocrocis me dinalis
摘  要:
The susceptibility of the neonates of rice lepidopterans,striped stem borer Chilo suppressalis(Walker),pink stem borer Sesamia inferens(Walker) and leaffolder,Cnaphalocrocis medinalis(Guenée) to four Cry proteins,i.e.Cry1Ab,Cry1Ac,Cry1C,and Cry2A,from Bacillus thuringiensis were bioassayed using diet-feeding method and leaf-immersion method.The LC50 values of Cry1Ab,Cry1Ac,Cry1C,and Cry2A to C.suppressalis were 1.739 ng/mg,2.645 ng/mg,2.794 ng/mg,and 15.965 ng/mg,respectively;and those to S.inferens were 2.455 ng/mg,10.046 ng/mg,11.803 ng/mg,and 35.359 ng/mg,respectively;and to C.medinalis,4.000μg/ml,1.611μg/ml,69.031μg/ml,and 12.708μg/ml,respectively.Finally,the difference in susceptibility of rice lepidopterans to Cry proteins and its application to transgenic insect-resistant crops were also discussed.

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