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Position: Home > Articles > Molecular Biological Identification of Active Dry Yeast for Grape Wine FOOD SCIENCE 2009,30 (19) 200-202

分子生物学方法区分鉴定五种常用葡萄酒活性干酵母

作  者:
李艳;卢君;崔建东;杨美景
单  位:
河北科技大学生物科学与工程学院
关键词:
活性干酵母;葡萄酒;区分鉴定;PCR-RFLP(聚合酶链式反应-限制性片段长度多态性);inter-δPCR
摘  要:
通过分子生物学方法对5种常用的葡萄酒活性干酵母进行区分鉴定。所用的分子手段为PCR-RFLP结合inter-δPCR的方法,将活性干酵母在亚种甚至菌株水平上进行区分,得到5种活性干酵母独特的inter-δPCR扩增图谱,并且预测扩增产物的分子量。
译  名:
Molecular Biological Identification of Active Dry Yeast for Grape Wine
作  者:
LI Yan1,2,LU Jun1,CUI Jian-dong1,2,YANG Mei-jing1 (1. College of Bioscience and Bioengineering,Hebei University of Science and Technology,Shijiazhuang 050018,China; 2. Research and Development Center for Fermentation Engineering of Hebei Province,Shijiazhuang 050018,China)
关键词:
active dry yeasts;grape wine;differentiate and identify;PCR-RFLP;inter-δPCR
摘  要:
Five commonly used active dry yeasts for grape wine were differentiated and identified by molecular biological techniques. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and inter-δ PCR were used to differentiate and identify at subspecies or even strains level. Five unique inter-δPCR amplification patterns were obtained and molecular sizes of amplified products were predicted.

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