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HPLC法测定咸蛋、皮蛋黄中的四种苏丹红染料

作  者:
王明月;桂卫星;袁宏球
单  位:
南昌市蔬菜科学研究所;中国热带农业科学院分析测试中心
关键词:
咸蛋;皮蛋;苏丹红Ⅰ;苏丹红Ⅱ;苏丹红Ⅲ;苏丹红Ⅳ;高效液相色谱(HPLC)
摘  要:
采用高效液相色谱(HPLC)法测定咸蛋、皮蛋中的四种苏丹红染料。测定结果表明,苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ在0~0.5mg/L时其峰面积与进样质量浓度呈良好的线性关系,其线性相关系数为0.9999、0.9995、0.9999和0.9999(n=6),加标样品测定的相对标准偏差(RSD)为1.1%、2.2%、1.9%和2.4%(n=6)。苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ的最小检出限均为10μg/kg。苏丹红Ⅰ、Ⅱ、Ⅲ、Ⅳ回收率均为90%~105%。结果显示:该方法快速、灵敏、可靠,具有简便、重现性好的特点。
译  名:
High Performance Liquid Chromatography for Determination of Sudan Dyes in Salted and Preserved Eggs
作  者:
WANG Ming-yue1,GUI Wei-xing2,YUAN Hong-qiu1 (1. Analysis and Testing Centre,Chinese Academy of Tropica Agricultural Sciences,Haikou 571101,China; 2. Nanchang Vegetable Science Research Institute,Nanchang 330001,China)
关键词:
salted egg;preserved egg;Sudan dye Ⅰ;Sudan dye Ⅱ;Sudan dye Ⅲ;Sudan dye Ⅳ; high performance liquid chromatography (HPLC)
摘  要:
The method for separation and determination of Sudan dyes in salted egg and preserved egg by reversed-phase high performance liquid chromatography was developed using chromatography conditions. The results showed that Sudan dyes have good linearity in the range from 0 to 0.5 mg/L,the correlation coefficients are 0.9999,0.9995,0.9999 and 0.9999 (n=6) respectively for four Sudan dyes. The relative standard deviations of spiked sample are 1.1%,2.2%,1.9% and 2.4% (n=6). The detection limits of all four Sudan dyes are 10 μg/ kg. The average recovery of four Sudan dyes is 95% to 105%. In conclusion,this method is rapid,sensitive,simple,accurate and reproducible.

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