中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 黑麦6R染色体特异性PCR标记的建立 麦类作物学报 2007,27 (1) 35-40
Position: Home > Articles > Establishment of a Specific PCR Marker of 6R Chromosome of Rye Journal of Triticeae Crops 2007,27 (1) 35-40

黑麦6R染色体特异性PCR标记的建立

作  者：
刘成;杨足君;冯娟;周建平;任正隆
单  位：
电子科技大学生命科学与技术学院
关键词：
黑麦;6R染色体;RAPD扩增;分子标记
摘  要：
为了筛选黑麦染色体组特异性分子标记,以荆州黑麦、秦岭黑麦、森林黑麦、非洲黑麦、普通小麦中国春、R25、R111和MY11为材料,用A-M组142个10碱基随机引物进行RAPD扩增。发现引物M04可以在所有黑麦中扩出一条长1 143 bp(经测序)的特异片段OPM041143,而供试小麦材料均未扩出该片段。根据OPM041143设计特异引物M4-F和M4-R。利用M4-F和M4-R对含黑麦染色体的材料和小麦族其它物种进行扩增,结果表明,只有含黑麦染色体的材料均可以扩出一条分子量为1 082 bp的DNA带,命名为PSCM1082。进而利用一套中国春-Imperial黑麦二体附加系和几个小麦-黑麦染色体6R衍生系进行扩增,发现仅含6R染色体的材料能扩增出PSCM1082,这说明PSCM1082是黑麦6R染色体所特有。黑麦6R染色体特异PCR标记PSCM1082的发现,对于快速跟踪检测导入小麦中的6R染色体具有实用价值,可以应用于小麦育种工作中。
译  名：
Establishment of a Specific PCR Marker of 6R Chromosome of Rye
作  者：
LIU Cheng,YANG Zu-jun,FENG Juan,ZHOU Jian-ping,REN Zheng-long (School of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054,China)
关键词：
Rye;6R chromosome;RAPD;Molecular marker
摘  要：
In order to find out special molecular marker of genome of rye,random amplified polymorphic DNA(RAPD) analysis was performed on Secale cereale,S.creale.L.Qingling,S.silvestre,S.africanum,common wheat cultivar Chinese Spring,R25,R111 and MY11 with 142 random 10-based primers.A chromosome-specific polymorphic DNA segment,OPM041143,was obtained in all of the rye derived lines,but there was no OPM041143 in all wheat materials tested in this study.A pair of special primers M4-F and M4-R was designed based on OPM041143 in order to obtain a sequence charactered amplified region(SCAR) marker.The SCAR-PCR was performed on materials containing rye chromosome and some other Triticeae species using M4-F and M4-R.The result showed that special DNA segment of 1 082 bp only obtained in all material containing rye chromosome,named PSCM1082 and there was no PSCM1082 in other material of Triticeae.Furthermore,PCR analysis was performed on a set of Chinese Spring-S.cereale addition lines and several wheat –rye 6R chromosome derivatives,the result showed that PSCM1082 could be amplified in all materials contain 6R chromosome.Therefore,PSCM1082 is a chromosome-specific polymorphic DNA segment for rye chromosome 6R,it could be used as a molecular marker for detection of 6R chromosome of rye in wheat.

相似文章

导出引用
计量
文章访问数: 15
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46