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Position: Home > Articles > Determination of Sudan Pigment in Preserved Egg by Combination of Matrix Solid Phase Dispersion and High Performance Liquid Chromatography FOOD SCIENCE 2008 (3) 400-402

基质固相分散-HPLC测定松花蛋中苏丹红

作  者:
刘宏程;王至飞;黎其万;邹艳红;邵金良;唐利斌
单  位:
农业部农产品质量监督检验测试中心
关键词:
基质固相分散;松花蛋;苏丹红I~IV;HPLC
摘  要:
建立松花蛋中苏丹红I~IV残留分析的高效液相色谱法。样品通过基质固相分散(MSPD)快速萃取净化,Novapak C18(3.9×250mm,5μm)进行分离,用双波长紫外检测器双波长进行检测,波长为478nm和520nm,对于阳性样品通过双柱进行确认。回收率在85.5%~97.5%,相对标准偏差为3.1%~5.1%,最低检测限为0.1μg/ml。
译  名:
Determination of Sudan Pigment in Preserved Egg by Combination of Matrix Solid Phase Dispersion and High Performance Liquid Chromatography
作  者:
LIU Hong-cheng1,WANG Zhi-fei2,LI Qi-wan1,ZOU Yan-hong1,SHAO Jin-liang1,TANG Li-bin3 (1.Supervision and Testing Center for Farm Product Quality, Ministry of Agriculture, Yunnan Academy of Agriculture Science, Kunming 650223, China;2.College of Chemistry and Biotechnology,Yunnan National University, Kunming 650091, China; 3.Kunming Institute of Physics, Kunming 650223, China)
关键词:
matrix solid phase dispersion;preserved egg;Sudan I~IV;HPLC
摘  要:
An analytic method for Sudan I~IV residues determination in preserved eggs by HPLC was developed. The sample was extracted and purified quickly by matrix solid phase dispersion (MSPD), separated by a Waters Nova-Park C18 (3.9mm×150 mm,5μm) and detected respectivey with two wavelengths UV detector at 478nm and 520 nm. Two pillars confirmed a positive result. The recoveries range of determination is 85.5%~97.5%. The relative standard deviation (RSD) is 3.1%~5.1%. The detection limit is 0.1μg/ml.

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