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Position: Home > Articles > The clone and sequence analysis on the CP gene of potato virus A Plant Protection 2003,29 (5) 25-28

马铃薯A病毒CP基因的克隆与序列分析

作  者:
吴兴泉;陈士华;吴祖建;林奇英;谢联辉
单  位:
福建农林大学植物病毒研究所
关键词:
基因工程;马铃薯A病毒;分子鉴定与检测
摘  要:
利用根据马铃薯A病毒 (PVA)外壳蛋白 (CP)基因序列设计合成的一对引物 ,以带毒植物总RNA为模板 ,RT-PCR扩增得到长 0 8kb的目的片段。将目的片段转入大肠杆菌并进行了序列测定。测序结果与PVA其他分离物CP基因序列比较 ,发现其核苷酸同源性最高可达 99%。依据CP序列建立了PVA病毒的系统进化树并对PVA不同分离物CP氨基酸序列差异性做了分析
译  名:
The clone and sequence analysis on the CP gene of potato virus A
作  者:
WU Xing-quan, CHEN Shi-hua, WU Zu-jian, LIN Qi-Ying, XIE Lian-hui (Plant Virology Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
关键词:
gene engineering; potato virus A; identification and detection
摘  要:
With the specific primers which were designed based on the potato virus A (PVA) coat protein (CP) gene sequence, one gene fragment (0.8 kb) was amplified by reverse transcription polymerasae chain reaction (RT PCR) using the total RNA of the plant infected by PVA. The gene was cloned into Escherichia coli DH5α and sequenced. The sequence was compared with the sequence of the homologous gene of other isolates of PVA The result showed it had high homology with the other isolates (the highest homology could reach 99% of nucleic acid). Based on CP amino acid sequence the phylogenic tree of PVS was established and the isolates were clustered into many groups.

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