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Position: Home > Articles > Isolation and identification of Lactococcu lactis producing nisin Journal of Zhejiang University (Agriculture and Life Sciences) 2005,31 (5) 645-648

乳链菌肽产生菌的筛选及其鉴定

作  者:
周绪霞;姚江涛;李卫芬
单  位:
浙江大学饲料科学研究所教育部动物分子营养学重点实验室
关键词:
乳酸乳球菌;乳链菌肽;筛选;鉴定
摘  要:
乳链菌肽产生菌中乳链菌肽编码基因、乳链菌肽抗性基因和蔗糖发酵基因紧密连锁.根据该原理,在添加乳链菌肽、蔗糖及溴甲酚紫的M17选择培养基上,从新鲜牛奶样品中定向筛选到6株能在选择培养基上生长的菌株,通过细菌生理生化指标鉴定、16S rDNA序列测定,以及发酵产物的抑菌试验,获得了两株产乳链菌肽的乳酸乳球菌和两株只对乳链菌肽具有抗性但不产生乳链菌肽的乳酸乳球菌.
译  名:
Isolation and identification of Lactococcu lactis producing nisin
作  者:
ZHOU Xu-xia~(1,2),YAO Jiang-tao~1,LI Wei-fen~1 (1.Institute of Feed Science,Zhejiang University,The Key Laboratory of Animal Molecular Nutrition,Ministry of education,Hangzhou 310029,China;(2.Deparment)of Chemistry,Zhejiang University,Hangzhou 310027,China)
关键词:
Lactococcus lactis;nisin;isolation;identification
摘  要:
In Lactococcus lactis strains producing nisin,nisin production is encoded by a large conjugative nisin-sucrose transposon including genes involved in immunity to nisin,and under the guidance of this principle,6 strains were isolated from fresh milk samples on M17 culture medium supplemented with nisin,sucrose and bromocresol.Further analysis of physiological and biochemical characteristics,16S rDNA nucleotide sequences of these strains and test for antimicrobial activities of their fermentation products revealed that,two nisin-producing strains and two strains that were resistant to but failed to produce nisin were obtained.

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