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当前位置: 首页 > 文章 > 白藜芦醇对晚期糖基化终末产物诱导INS-1细胞损伤的保护作用及机制 食品工业科技 2018 (24) 145-150
Position: Home > Articles > Protective Effect and Mechanism of Resveratrol on INS-1 Cell Injury Induced by Advanced Glycation End Products Science and Technology of Food Industry 2018 (24) 145-150

白藜芦醇对晚期糖基化终末产物诱导INS-1细胞损伤的保护作用及机制

作  者：
郑晓茂;张汉语;陈琳;茹琴;李超英
单  位：
太和医院临床营养科;江汉大学武汉生物医学研究院
关键词：
晚期糖基化终末产物;白藜芦醇;INS-1细胞;抗氧化应激;抗炎
摘  要：
目的:研究白藜芦醇(RSV)对晚期糖基化终末产物(AGEs)诱导大鼠胰岛素瘤细胞(INS-1)损伤的保护作用及其抗炎抗氧化应激机制。方法:本实验使用含AGEs的糖化血清(GS)建立INS-1细胞损伤模型。实验分对照组、20%GS模型组、20%GS+不同浓度RSV组。检测各组细胞增殖率、细胞内超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)含量、培养液上清一氧化氮(NO)和细胞内活性氧(ROS)、丙二醛(MDA)含量以及培养液上清肿瘤坏死因子(TNF-α)含量。结果:与对照组比较,模型组INS-1细胞增殖率、SOD活性和GSH的含量极显著降低(p <0.01),NO和ROS含量极显著升高(p <0.01),MDA含量显著升高(p <0.05),TNF-α含量极显著降低(p <0.01)。与模型组比较,25和50μmol/L的RSV组INS-1细胞增殖率均极显著提高(p <0.01),同时SOD活力和GSH含量极显著升高(p <0.01),NO的水平极显著降低(p <0.01)。此外,50μmol/L的RSV组ROS含量显著降低(p <0.05),25μmol/L的RSV组MDA含量显著降低(p <0.05),25和50μmol/L的RSV组的TNF-α水平极显著降低(p <0.01)。结论:RSV对AGEs损伤的INS-1细胞有显著的保护作用,其作用机制可能是通过提高INS-1细胞的抗氧化应激能力和抗炎症反应能力实现的。
译  名：
Protective Effect and Mechanism of Resveratrol on INS-1 Cell Injury Induced by Advanced Glycation End Products
作  者：
ZHENG Xiao-mao;ZHANG Han-yu;CHEN Lin;RU Qin;LI Chao-ying;Wuhan Institutes of Biomedical Sciences of Jianghan University;Clinical Nutrition Department of Taihe Hospital;
单  位：
ZHENG Xiao-mao%ZHANG Han-yu%CHEN Lin%RU Qin%LI Chao-ying%Wuhan Institutes of Biomedical Sciences of Jianghan University%Clinical Nutrition Department of Taihe Hospital
关键词：
advanced glycation end products;;resveratrol;;INS-1-cells;;anti-oxidative stress;;anti-inflammatory
摘  要：
Objective: The aim of this study was to investigate the protective effect of resveratrol (RSV) on the rat INS-1 cell injury induced by advanced glycation end products (AGEs) and the underlying mechanisms of anti-inflammatory and anti-oxidative stress involved in it.Methods: In this experiment,the glycation serum (GS) which included various AGEs was used to mimic the diabetes settings and to establish a cell injured model.INS-1 cells were divided into control group,20% GS model group,20% GS + RSV groups with different concentrations. The cell proliferation rates was detected by MTT assay. The intracellular SOD activities,GSH contents,supernatant NO,intracellular ROS,MDA contents and supernatant TNF-α were measured by Kits.Results: Compared with control group,the model group cells proliferation rate were significantly decreased (p < 0.01). The SOD activities and the levels of GSH in model group were significantly decreased (p < 0.01).The levels of NO and ROS in model group were significantly increased (p < 0.01).The levels of MDA in model group were significantly increased (p <0.05). The levels of TNF-α in model group were significantly increased (p < 0.01). Compared with model group,the proliferation rates of INS-1 cells of 25 and 50 μmol/L RSV group were significantly increased (p < 0.01),and the content of GSH and SOD activities were significantly increased (p < 0.01),the contents of NO were significantly decreased (p < 0.01).In addition,the levels of MDA were significantly reduced (p < 0.05) in 25 μmol/L RSV group and the levels of ROS were significantly reduced (p < 0.05) in 50 μmol/L RSV group. The contents of TNF-α were significantly decreased (p < 0.01).Conclusion: RSV has a significant protective effect on the AGEs injured INS-1 cells,and the mechanism would be related to improve the anti-oxidative stress and anti-inflammatory abilities of INS-1 cells.

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