当前位置: 首页 > 文章 > 荆江麻鸭GH基因第4外显子SNP及其与屠体性状的关联研究 中国家禽 2011,33 (14) 32-35
Position: Home > Articles > Study on Association between Single Nucleotide Polymorphism of Exon4 of GH Gene with Carcass Traits in Jingjiang Partridge Duck China Poultry 2011,33 (14) 32-35

荆江麻鸭GH基因第4外显子SNP及其与屠体性状的关联研究

作  者:
杨军;罗家琴;唐登华;李助南
单  位:
长江大学动物科学学院
关键词:
荆江麻鸭;GH基因;屠体性状;关联分析
摘  要:
采用PCR-SSCP方法检测了96只荆江麻鸭生长激素基因第4外显子序列多态性,并分析了基因多态性与屠体性状间的关系。结果发现,在荆江麻鸭生长激素基因第4外显子中检测到1个突变位点,为3701nt处C→T碱基转换,产生AA、BB和AB3种基因型,其中野生基因型BB为优势基因型(54.17%)。卡方检验结果表明,群体该位点处于遗传平衡状态(P>0.05)。最小二乘分析结果表明,AA基因型个体全净膛率显著高于BB基因型(P<0.05)。初步推断,GH基因可作为影响荆江麻鸭部分屠体性状的候选基因(标记),其中突变基因型AA为有利基因型。
译  名:
Study on Association between Single Nucleotide Polymorphism of Exon4 of GH Gene with Carcass Traits in Jingjiang Partridge Duck
作  者:
YANG Jun,LUO Jiaqin,TANG Denghua,LI Zhunan(College of Animal Science,Changjiang University,Jingzhou,Hubei 434025)
关键词:
Jingjiang partridge duck;growth hormone gene;carcass traits;association analysis
摘  要:
Polymorphism of GH gene exon4 was identified by PCR-SSCP method in 96 Jingjiang partridge ducks,and association of polymorphism with carcass traits was investigated.A cytosine to thymine transition mutation at 3 701 nt was detected.Three genotypes of AA,AB and BB were classified and wild genotype BB was dominant in population(54.17%).Results of Chi-square test indicated that this site was in Hardy-Weinberg equilibrium in Jingjiang partridge duck(P>0.05).The results of least square analysis showed that ducks with AA genotype had higher percentage of halfevisceration than those of BB ducks(P<0.05).It was concluded that GH gene could be regarded as a useful candidate gene(marker) for part of carcass traits of Jingjiang partridge duck,in which mutant AA genotype was considered as advantageous genotype.

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