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Position: Home > Articles > Screening and identifying Lactobionic Acid producing bacteria strain Science and Technology of Food Industry 2013 (4) 189-192

乳糖酸生产菌株的筛选与鉴定

作  者:
郑艳;李超
单  位:
沈阳农业大学食品学院
关键词:
乳糖酸;生产菌株;筛选;鉴定
摘  要:
乳糖酸具有良好的功能特性。经富集培养,从土壤样品中分离出了201株菌株,经x-gal平板培养基、伊红美蓝合成培养基和指示平板培养基筛选出29株疑似乳糖酸生产菌株。利用薄层色谱法定性鉴定出5株疑似乳糖酸生产菌株,最终用高效液相色谱法确定出一株优良的乳糖酸生产菌株Y20,其乳糖酸产量为127.65g/L,转化率为85.10%,经生理生化鉴定和16SrDNA分子鉴定,该菌为土生拉乌尔菌。
译  名:
Screening and identifying Lactobionic Acid producing bacteria strain
作  者:
ZHENG Yan,LI Chao(College of Food Science,Shenyang Agricultural University,Shenyang 110866,China)
关键词:
Lactobionic Acid;producing strain;screening;identification
摘  要:
Lactobionic Acid has good function characteristics.Through enrichment,201 strains were isolated and 29 suspect Lactobionic Acid producing bacteria strains were screened through x-gal,EMB and instruction plate medium.Then 5 suspect Lactobionic Acid producing strains were got by using TLC qualitative identification.Finally a good Lactobionic Acid producing strain No.20 was obtained through HPLC.The production of Lactobionic Acid was 127.65g/L and conversion ratio was 85.10%.Through physiology and biochemistry identification and 16SrDNA molecular identification,the strain was Raoultella terrigena.

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