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当前位置: 首页 > 文章 > 河川沙塘鳢(♀)×鸭绿沙塘鳢(♂)双亲及其杂交子代的核型分析 海洋渔业 2013,35 (2) 183-188
Position: Home > Articles > Karyotypes of Odontobutis potamophila(♀)×Odontobutis yaluensis(♂) and their hybrid progeny Marine Fisheries 2013,35 (2) 183-188

河川沙塘鳢(♀)×鸭绿沙塘鳢(♂)双亲及其杂交子代的核型分析

作  者：
张丽娟;祝斐;尹绍武;侯新远;胡亚丽
单  位：
江苏省生物多样性与生物技术重点实验室;南京师范大学生命科学学院
关键词：
河川沙塘鳢;鸭绿沙塘鳢;杂交子代;核型
摘  要：
采用人工授精法以河川沙塘鳢(Odontobutis potamophila)为母本、鸭绿沙塘鳢(O.yaluensis)为父本进行杂交,获得正常发育的杂交子代。利用胸腔活体注射植物凝集素(PHA)和秋水仙素溶液后,取头肾细胞进行低渗处理,空气干燥法制片,对河川沙塘鳢、鸭绿沙塘鳢及其杂交子代的核型进行了比较研究。结果表明:河川沙塘鳢的核型为2n=44,2st+42t,NF=44;鸭绿沙塘鳢的核型为2n=44,2st+42t,NF=44;杂交子代的核型为2n=44,4st+40t,NF=44。经对比分析,杂交子代分别继承了双亲的一套染色体,表明杂交子代为真正的杂交种。
译  名：
Karyotypes of Odontobutis potamophila(♀)×Odontobutis yaluensis(♂) and their hybrid progeny
作  者：
ZHANG Li-juan,ZHU Fei,YIN Shao-wu,HOU Xin-yuan,HU Ya-li(Key Laboratory of Biodiversity and Biotechnology of Jiangsu Province,College of Life Sciences, Nanjing Normal University,Nanjing 210023,China)
关键词：
Odontobutis potamophila;Odontobutis yaluensis;hybrid progeny;karyotype
摘  要：
The progenies of distant hybridization between Odontobutis potamophila(♀) and O.yaluensis(♂) were successfully obtained by artificial insemination.The metaphase chromosomes of O.potamophila(♀),O.yaluensis(♂) and their progenies were obtained from head kidney tissue by the method of PHA,colchicines injection and hypotonic-air drying technique.The results showed that the diploid chromosome number of O.potamophila(♀) was 2n=44,karyotype formula was 2st+42t and arm number(NF)=44.The diploid chromosome number of O.yaluensis(♂) was 2n=44,karyotype formula was 2st+42t and arm number(NF)=44.The diploid chromosome number of their progenies were 2n=44,karyotype formula was 4st+40t and arm number(NF)=44.By comparison with the chromosome karyotype of O.potamophila(♀),O.yaluensis(♂) and their progeny,it could be concluded that the progeny inherited one set of chromosomes from their parents,respectively.Therefore,the progeny was indeed hybrid species.

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