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胞外单宁酶纯化及酶学性质的研究

作  者:
王维维;邱树毅;谢晓莉;吴鑫颖;李广神
单  位:
贵州大学化学与化工学院;贵州省发酵工程与生物制药重点实验室;贵州大学化学与化工学院贵州遵义高等医科专业学校贵州省发酵工程与生物制药重点实验室
关键词:
单宁酶;生料发酵;纯化
摘  要:
采用黑曲霉B0201固态生料发酵生产胞外单宁酶,经过硫酸铵分级沉淀、DEAE-纤维素层析、葡聚糖G-150凝胶柱层析纯化,得到电泳纯的单宁酶,纯化倍数达到18.44。对该酶的性质研究表明:单宁酶的相对分子质量大小约为58800,该酶的最适反应温度是40℃,最适反应pH值是5.0;以PG为底物,米氏常数Km为1.08mmol/L,Vmax为104.1μmol/(L.min)。
译  名:
Purification and Enzymatic Properties of Extracellular Tannase from Aspergillus niger
作  者:
WANG Wei-wei1,2,QIU Shu-yi1,2,,XIE Xiao-li1,2,WU Xin-ying3,LI Guang-shen2,3,HE Shun-rong2,3(1.College of Life Science,Guizhou University,Guiyang 550025,China; 2.Guizhou Province Key Laboratory of Fermentation and Biopharmacy,Guiyang 550003,China; 3.School of Chemistry and Chemical Engineering,Guizhou University,Guiyang 550003,China)
关键词:
tannase;fermentation without cooking;purification
摘  要:
Extraction with sodium citrate-citric acid buffer,(NH4)2SO4 fractional precipitation,DEAE-cellulose column separation and Sephadex G-150 column purification were sequentially carried out to obtain an electrophoretically pure extracellular tannase from solid state cultures of Aspergillus niger B0201,with a purification fold of 18.44.The enzyme had a molecular weight of roughly 58800 and the optimum reaction temperature and pH were 40 ℃ and 5.0,respectively.When the substrate was propyl gallate(PG),the Km was 1.08 mmol/L and the Vmax 104.1μmol/(L·min).

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