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Position: Home > Articles > Study on screening and induction conditions of the tannase producing fungi Journal of Nanjing Forestry University(Natural Sciences Edition) 2010,34 (3) 10-14

产单宁酶真菌的筛选及产酶条件

作  者:
谷文;杨亦陈;王石发
单  位:
南京林业大学化学工程学院
关键词:
单宁酶;筛选;发酵条件;绿色木霉
摘  要:
从铁冬青等4种植物的新鲜茎、叶组织中分离得到52株真菌,经平板透明圈法初筛得到18株产单宁酶菌株。初筛所得菌株经液体发酵,显示1株木霉属真菌(Trichoderma viride)(No.MY P07)酶活力最高,达0.248μmol/(min·mL)。采用单因素结合正交实验,得到菌株MYP07的最佳发酵条件为:单宁100g/L,蔗糖10g/L,(NH4)2SO43g/L,装液量为30mL(250mL锥形瓶),起始pH为5.0,温度30℃,转速140r/min。在此条件下,菌株MY-P-07发酵48h酶活力达到0.392μmol/(min·mL)。
译  名:
Study on screening and induction conditions of the tannase producing fungi
作  者:
GU Wen,YANG Yi-chen,WANG Shi-fa (College of Chemical Engineering,Nanjing Forestry University,Nanjing 210037,China)
关键词:
tannase; screening; fermentation condition; Trichoderma viride
摘  要:
Fifty-two strains of filamentous fungi were isolated from the fresh stems and/or leaves of 4 plants,and 18 tannase producing strains were obtained through the plate transparent zone method. Subsequently these strains were cultured in the liquid tannase-inducing medium,among which a fungal strain Trichoderma viride (No. MY P 07) showed the strongest activity (0. 248 μmol/(min·mL)). Through single factor and orthogonal experiments,the optimal fermentation conditions of the strain MY P 07 were determined as follows:tannin acid 100 g/L,sucrose 10 g/L,(NH4)2SO4 3 g/L,liquid volume 30 mL (in 250 mL flasks),initial pH 5. 0,30 ℃,140 r/min for 48 h. In these conditions,the tannase activity of the strain was 0. 392 μmol/(min·mL).

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