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HPLC法测定大豆磷脂中卵磷脂含量

作  者:
何新霞;郑孝华;郏海燕;叶锋
单  位:
浙江师范大学生物系
关键词:
大豆磷脂;卵磷脂;高效液相色谱法
摘  要:
应用Lichrosorb si60(5 m)250 4mm。色谱柱,乙腈:甲醇:水(65:21:14V/V)h流动相外标法测定了大豆磷脂中卵磷脂含量、卵磷脂检测线性范围为0.04-0.8mg/ml。r=0.9996,方法的回收率为98.35%,RSD为6.94% (n=6)。
译  名:
Quantitative Analysis of Lecithin in Phospholipids by HPLC
作  者:
He Xinxia et al
关键词:
Soybean Phospholipid lecithin HPLC
摘  要:
A simple rapid and accurate quantitative method was established for the determination of lecithin in soybean phospholipids by HPLC.Separation was obtained by using Lichrosorb si60 column (5 m250 x 4mm ) and mobile phase of acetonitrile:methanol:water (65:21:14v/v) with flow rate of 1.8ml/min .The eluates were monitored with UV detector at 205nm.The retention time for lecithin was 4.875min .The lecithin curve was linear in the range of 0.04 0.08mg/ml with =0.9996.The average recovery rate for lecithin was 98.35%.RSD=6.94%(n=6).

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