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当前位置: 首页 > 文章 > 食源性单增李斯特菌LPXTG蛋白基因的检测 中国兽医杂志 2017 (8) 17-21+25
Position: Home > Articles > Detection of LPXTG protein genes in Food-borne Listeria monocytogenes Chinese Journal of Veterinary Medicine 2017 (8) 17-21+25

食源性单增李斯特菌LPXTG蛋白基因的检测

作  者：
丁剑;马勋;陈朔;曹树珠;王贝贝;杜冬冬;张奇文
单  位：
石河子大学动物科技学院
关键词：
单核细胞增多性李斯特菌;LPXTG基序表面蛋白;PCR
摘  要：
单核细胞增多性李斯特菌(Listeria monocytogenes,LM)作为四大重要食源性病原菌之一,对人类及多种畜禽健康威胁极大。LM细胞胞壁表面分布多种毒力蛋白,在感染机体过程中发挥重要作用,其中有一类至关重要的表面蛋白其C末端含有保守基序LPXTG,分选酶A能够识别基序LPXTG,共价结合到肽聚糖上,呈现在细胞表面发挥毒力作用。本试验以不同食品中分离得到的24株单增李斯特菌为试验对象,设计合成了41对预测含LPXTG基序表面蛋白的引物,利用PCR技术进行扩增,凝胶电泳成像系统检测扩增产物片段大小,计算24株食品源单增李斯特菌LPXTG基序表面蛋白的携带。结果表明,不同LM分离株LPXTG蛋白基因的携带率不同:其中仅有12.5%(3/24)的分离株携带率在85%以上;不同LPXTG蛋白基因的检出率不同,在41个检测对象中,有8个基因的检出率100%,有3个基因的检出率不足20%,Lmo1115基因在所有分离株中均未检测到。本研究对了解食品源LM分离株的LPXTG蛋白基因的分布以及探明食品源LM的致病性具有重要意义。
译  名：
Detection of LPXTG protein genes in Food-borne Listeria monocytogenes
作  者：
DING Jian;MA Xun;CHEN Shuo;CAO Shu-zhu;WANG Bei-bei;DU Dong-dong;ZHANG Qi-wen;Shihezi University School of Animal Science and Technology;
单  位：
Shihezi University School of Animal Science and Technology
关键词：
Listeria monocytogenes;;LPXTG motif surface protein;;PCR
摘  要：
Listeria monocytogenes(Lm) is well known as one of the most important foodborne pathogens, posing a great threat tothe health of human and various livestock and poultry. Lm has a variety of virulence-associated proteins that are closely involved inthe pathogenicity. One type of the surface proteins which have a conserved LPXTG motif at COOH-terminal cell can be cleaved bysortase and anchored peptidoglycan to present on the cell surface to play a role in virulence. Twenty-four food-borne Lm strains wereisolated from different foods. Forty-one primers were designed and synthesized for putative LPXTG surface proteins. The geges weredetected by PCR and gel electrophoresis imaging system. The results showed that the rate of carrying LPXTG protein genes in differ-ent LM isolates was different. And 12. 5% Lm isolates(3/24)carried more than 85% LPXTG protein genes. The detection rate ofLPXTG protein genes was different. Among the 41 LPXTG protein genes, only 7 genes were 100% detected; 3 genes were under20%; the Lmo1115 gene was not detected. It is of importance for studying the pathogenicity of food-borne Lm to test the distribu-tion of LPXTG motif surface proteins.

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