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当前位置: 首页 > 文章 > 食源性单增李斯特菌LIPI-4基因的检测及序列分析 中国兽医学报 2019 (12) 2380-2385
Position: Home > Articles > Detection and sequence analysis of LIPI-4 gene on food-borne Listeria monocytogenes Chinese Journal of Veterinary Science 2019 (12) 2380-2385

食源性单增李斯特菌LIPI-4基因的检测及序列分析

作  者：
刘扬扬;康立超;马勋;李红欢;钱凌霄;李庆辉
单  位：
新疆农垦科学院分析测试中心;石河子大学动物科技学院
关键词：
单核细胞增多性李斯特菌;LIPI-4;PTS~(Lac)家族;PCR
摘  要：
单核细胞增多性李斯特菌(Listeria monocytogenes,LM)作为四大重要食源性病原菌之一,对公共安全及畜牧业发展威胁极大。其毒力岛4(LIPI-4)是新发现的一个潜在毒力因子,与LM的神经感染和母胎感染密切相关。以54株食源性LM的DNA为模板,针对LIPI-4的6段基因设计特异性引物,利用PCR的方法检测LIPI-4的携带情况并对携带株的LIPI-4基因进行生物信息学分析。结果表明,54株LM中有5株携带LIPI-4基因,携带率为9.26%;5株食源性LM LIPI-4基因的同源性均为100%,与CC1代表株LM09-00558 LIPI-4基因的同源性99%以上;对LIPI-4中EIIC结构域分析表明5株食源性LM LIPI-4均为PTS~(Lac)家族。食源性LM携带LIPI-4基因且属于PTS~(Lac)家族,研究LIPI-4基因对进一步了解LM的致病性具有重要意义。
译  名：
Detection and sequence analysis of LIPI-4 gene on food-borne Listeria monocytogenes
作  者：
LIU Yang-yang;KANG Li-chao;MA Xun;LI Hong-huan;QIAN Ling-xiao;LI Qing-hui;College of Animal Science and Technology,Shihezi University;Analysis and Testing Center of Xinjiang Academy of Agricultural and Reclamation Science;
关键词：
Listeria monocytogenes;;LIPI-4;;PTS~(Lac)family;;PCR
摘  要：
Listeria monocytogenes(LM) is well known as one of the four important food-borne pathogens,poses a great threat to public safety and animal husbandry.Listeria pathogenicity island 4(LIPI-4) is a newly discovered potential virulence factor,which is closely related to LM nerve infection and placental infection.Specific primers were designed and synthesized for 6 genes of LIPI-4,and these genes were detected by PCR from 54 food-borne LM strains isolated from the different foods.The sequence of LIPI-4 was analyzed by bioinformatics software.The results showed that 5 of 54 strains of LM carried LIPI-4 gene with a carrying rate of 9.26%;the homology of 5 food-borne LM LIPI-4 genes were 100%,and was more than 99% compared with that of LM09-00558 which was the representative strain of CC1.The analysis of EIIC domain in LIPI-4 showed that the LIPI-4 of five LM strains were all PTS~(Lac) family;sequence analysis of the the EIIC domain of LIPI-4 gene indicated the LIPI-4 of 5 strains of LM were all PTS~(Lac)(EIIC) family.LIPI-4 gene was detected from food-borne LM of Xinjiang and was belong to the PTS~(Lac) family.The study of LM LIPI-4 is important for further understanding of the pathogenicity of LM.

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