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当前位置: 首页 > 文章 > 三斑海马蛋白肽ACE抑制活性的研究 食品工业科技 2015,36 (15) 96-99
Position: Home > Articles > Angiotensin I- converting enzyme( ACE) inhibitory activity of enzymatic hydrolysate from three- spot hippocampus Science and Technology of Food Industry 2015,36 (15) 96-99

三斑海马蛋白肽ACE抑制活性的研究

作  者：
徐克寒;申铉日;陈国华
单  位：
海南大学海洋学院;海南大学食品学院
关键词：
三斑海马;ACE抑制;酶解液
摘  要：
本文通过三斑海马酶解液的制备、ACE抑制活性肽的分离和体外消化模型的建立研究了三斑海马蛋白肽的ACE抑制活性。利用碱性蛋白酶制备得到具有ACE抑制活性的三斑海马酶解液,经葡聚糖凝胶层析分离纯化后,得到具有较高ACE抑制活性的组分(其IC50为0.91 mg/m L);通过对该组分的氨基酸组成和体外消化模型分析,发现该组分中疏水性氨基酸的含量为50.48%,消化酶作用后,显著提高ACE抑制活性,经判断,该组分中的蛋白肽为前体型抑制剂。
译  名：
Angiotensin I- converting enzyme( ACE) inhibitory activity of enzymatic hydrolysate from three- spot hippocampus
作  者：
XU Ke-han;SHEN Xuan-ri;CHEN Guo-hua;College of Food Science and Technology,Hainan University;College of Ocean,Hainan University;
关键词：
three-spot hippocampus;;ACE-inhibitory activity;;hydrolysate
摘  要：
In this paper,the ACE- inhibitory activity of three- spot hippocampus peptides were evaluated by studying the preparation technique of three- spot hippocampus enzymatic hydrolysate,screening chromatography separation of ACE- inhibitory peptides and establishing the model.The enzymatic hydrolysate with ACE- inhibitory activity was prepared by alkaline protease,and then separated and purified by gel filtration chromatography.After determination,the lower molecular fraction exerted the highest ACE- inhibitory activity( IC50 was 0.91 mg / m L). The composition of amino acids in it was analyzed,and the test result indicated that the content of hydrophobic amino acids was 50.48%. In addition,it was suggested that the ACE- inhibitory peptides acted as ‘prodrug- type 'inhibitor after establishing the model of artificial gastrointestinal digestion. ACE- inhibitory activity was increased significantly under condition of digestive enzyme digestion.

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