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Position: Home > Articles > The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana Journal of Integrative Agriculture 2013,12 (3)

The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

作者：

Jiang Wei-yu;Geng Li-li;Dai Ping-li;Lang Zhi-hong;Shu Chang-long;Lin Yi;Zhou Ting;Song Fu-ping;Zhang Jie

单位：

Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;Huaqiao Univ, Coll Chem Engn, Xiamen 361021, Peoples R China;Chinese Acad Agr Sci, Key Lab Pollinating Insect Biol, Minist Agr, Inst Apicultural Res, Beijing 100093, Peoples R China;Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

关键词：

Bacillus thuringiensis;intestinal bacteria;horizontal gene transfer;transgenic maize;Apis cerana;biosafety

摘要：

Using culture-independent technique polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and Conventional culture techniques, ecological risk of transgenic maize pollen on gut bacteria of the Chinese honeybee, Apis cerana cerana, was assessed. Honeybees were fed with Bt-transgenic maize pollen, non-transgenic near isoline pollen, linear cry1Ah gene (800 ng mL(-1)) and supercoiled plasmid DNA (800 ng mL(-1)) under laboratory conditions. The DGGE profile showed that the number of DGGE bands varied from 10.7 to 14.7 per sample, and the Shannon's index ranged from 0.85 to 1.00. The similarity calculated by PAST was mostly above 92%, indicating no obvious changes among treatments or within replicates. 14 bacterial strains affiliated with Alphaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria were isolated and characterized on media under aerobic and anaerobic conditions. These results demonstrated that transgenic cry1Ah maize pollen did not induce significant changes of the honeybee gut bacterial community composition under laboratory conditions.

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The Influence of Bt-Transgenic Maize Pollen on the Bacterial Diversity in the Midgut of Chinese Honeybees, Apis cerana cerana

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