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Position: Home > Articles > The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36^I and 605^I Journal of Integrative Agriculture 2014,13 (5)

The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36^I and 605^I

作者：

Zhou Zi-shan;Lin Hui-yan;Li Ying;Shu Chang-long;Song Fu-ping;Zhang Jie

单位：

Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

关键词：

Bacillus thuringiensis;Cry1Ai toxin;active fragment;toxicity;Plutella xylostella

摘要：

The novel ciy1Ai gene that cloned from Bacillus thuringiensis strain SC6H8 encoded a protein exhibiting strong toxicity against Plutella xylostella and Chilo suppressalis in our previous study. Using the available information for the active fragments of other Cry toxins, eight truncated fragments were constructed to identify the minimal active fragment of Cry1Ai. All truncated fragments were expressed in Escherichia coli strain BL21 (DE3), and the insecticidal activity against 2nd-instar P. xylostella larvae was assessed using full-length Cry1Ai as a positive control. The results indicate that the minimal active fragment of the Cry1Ai toxin against P. xylostella is located between amino acid residues 36(I) and 605(I), which is smaller than the regions previously reported for Cry1A. The first two amino acids (34(T) and 35(P)) on helix alpha-1 and whole helix alpha-2 of domain I and sheet beta-32 of domain III are necessary for Cry1Ai toxin to keep its toxicity against P. xylostella.

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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36^I and 605^I

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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36I and 605I

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The Minimal Active Fragment of the Cry1Ai Toxin is Located Between 36^I and 605^I