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Position: Home > Articles > Determination of Benzo(a)pyrene in Meat Products by HPLC with Solid-Phase Extraction MEAT RESEARCH 2012 (6) 37-40

固相萃取-高效液相色谱法测定肉制品中苯并(a)芘

作  者：
林慧;颜春荣;徐春祥;卢剑
单  位：
江苏省产品质量监督检验研究院
关键词：
苯并(a)芘;肉制品;固相萃取;高效液相色谱法
摘  要：
建立固相萃取-高效液相色谱法测定肉制品中苯并(a)芘的检测方法。样品经Cleanert BaP固相萃取柱净化、浓缩,Waters SunFire C18(4.6mm×250mm,5μm)色谱柱进行分离,流动相为乙腈-水(88:12,V/V),流速1.0mL/min,荧光检测器,激发波长386nm,发射波长405nm。结果表明,苯并(a)芘在线性范围0.5～10ng/mL内,相关系数为0.9978,最低检出限为0.04μg/kg,精密度(RSD)小于5%,回收率82.2%～94.9%。本方法快速简便、重现性好,可以用于肉制品中苯并(a)芘含量的测定,为肉制品的质量控制和安全评价提供保证。
译  名：
Determination of Benzo(a)pyrene in Meat Products by HPLC with Solid-Phase Extraction
作  者：
LIN Hui,YAN Chun-rong,XU Chun-xiang,LU Jian(Jiangsu Institute for Product Quality Supervision and Inspection,Nanjing 210007,China)
关键词：
benzo(a)pyrene;meat products;solid-phase extraction;high performance liquid chromatography(HPLC)
摘  要：
A high performance liquid chromatography with solid-phase extraction method was developed for the determination of benzo(a)pyrene in meat products.The extracted sample was purified on a Cleanert BaP solid-phase extraction column and separated on a Waters SunFire C18 column(4.6 mm × 250 mm,5μm) using a mobile phase composed of acetonitrile and water(88/12) at a flow rate of 1.0 mL/min.Benzo(a)pyrene was measured using a fluorescence detector at an excitation wavelength of 386 nm and an emission wavelength of 405 nm.The result showed that benzo(a)pyrene displayed good linearity relationship over the concentration range of 0.5-10 ng/mL,with a coefficient correlation of 0.9978.The limit of detection was 0.04 μg/kg,and the precision(RSD) for 6 replication determinations was below 5%.The average blank spike recoveries across the levels was in the range of 82.2%-94.9%(n=3).This method was rapid,simple,reproducible,and applicable for benzo(a)pyrene determination in meat products,providing guarantee for quality control and safety evaluation of meat products.

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