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Position: Home > Articles > Determination of Carrageenan in Livestock Meat by High Performance Liquid Chromatography-Mass Spectrometry FOOD SCIENCE 2019 (10) 298-303

高效液相色谱-质谱法测定畜肉中的卡拉胶

作  者：
张会亮;黄传峰;李佩璇;江丰;孙姗姗;丁宏;曹进
单  位：
中国食品药品检定研究院;临沂市食品药品检验检测中心;湖北省食品质量安全监督检验研究院
关键词：
注胶肉;卡拉胶;寡糖;高效液相色谱-质谱法;食品安全
摘  要：
建立测定畜肉中卡拉胶的高效液相色谱-质谱法。生鲜肉样品用1 mol/L盐酸处理,其中的卡拉胶降解生成[A-G4S]-等特征性寡糖片段,采用高效液相色谱-三重四极杆质谱进行定性和定量测定。可选择高分辨质谱用于确证。结果表明,对于正常畜肉样品不出现假阳性,对于标识添加卡拉胶的调理牛排等阳性样品均能有效识别。对于m/z 403>241离子对,检出限为20 mg/kg(卡拉胶/肉),线性范围为0.1～0.4 g/kg,线性相关系数在0.99以上,相对回收率为90%～120%。该方法前处理简便、检测指标明确、灵敏度高,可作为检测添加卡拉胶畜肉的有效手段。
译  名：
Determination of Carrageenan in Livestock Meat by High Performance Liquid Chromatography-Mass Spectrometry
作  者：
ZHANG Huiliang;HUANG Chuanfeng;LI Peixuan;JIANG Feng;SUN Shanshan;DING Hong;CAO Jin;National Institutes for Food and Drug Control;Linyi Food and Drug Inspection and Testing Center;Hubei Provincial Institute for Food Supervision and Test;
单  位：
ZHANG Huiliang%HUANG Chuanfeng%LI Peixuan%JIANG Feng%SUN Shanshan%DING Hong%CAO Jin%National Institutes for Food and Drug Control%Linyi Food and Drug Inspection and Testing Center%Hubei Provincial Institute for Food Supervision and Test
关键词：
gum-injected meat;;carrageenan;;oligosaccharide;;high performance liquid chromatography-mass spectrometry(HPLC-MS);;food safety
摘  要：
This study aimed to establish a method for the determination of added carrageenan in livestock meat by high performance liquid chromatography-mass spectrometry(HPLC-MS). Carrageenan in fresh meat was digested with 1 mol/L hydrochloric acid, yielding characteristic oligosaccharide fragments such as [A-G4 S]-, which were detected by HPLC-triple quadrupole mass spectrometry. A high resolution mass spectrometer was employed for validation of the method. The results showed that no false positive results were found for natural meat samples, while positive samples such as steak labelled to contain carrageenan were identi?ed effectively by the proposed method. The limit of detection(LOD) of carrageenan was20 mg/kg(at m/z 403 > 241). A good linear relationship was observed in the concentration range of 0.1–0.4 g/kg with correlation coef?cients of more than 0.99. The relative recoveries were in the range of 90%–120%. In summary, this method, thanks to its simplicity, clear target and high sensitivity, could be used as an effective means to detect carrageenan-injected livestock meat.

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