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当前位置: 首页 > 文章 > 不同温度下福氏志贺菌与其膜囊泡的比较蛋白质组学研究 云南农业大学学报(自然科学) 2017 (6) 967-974
Position: Home > Articles > Comparative Proteomic Analysis of Shigella flexneri and Its Outer Membrane Vesicle Journal of Yunnan Agricultural University(Natural Science) 2017 (6) 967-974

不同温度下福氏志贺菌与其膜囊泡的比较蛋白质组学研究

作  者：
韩冬梅;卫灿东
单  位：
中国医学科学院病原生物学研究所
关键词：
福氏志贺菌;膜囊泡;蛋白质组;三型分泌系统
摘  要：
【目的】对不同温度下福氏志贺菌(Shigella flexneri)及其分泌的膜囊泡进行比较蛋白质组学分析,为进一步理解福氏志贺菌膜囊泡的生物学活性奠定基础。【方法】采用差速离心和密度梯度离心方法,分别富集纯化37℃和30℃培养条件下的野生型福氏志贺菌2a 301株和相应的膜囊泡;借助二维纳升液相色谱-线性离子阱质谱联用系统,对制备得到的4种组分蛋白进行鸟枪蛋白质组学鉴定分析。【结果】37℃和30℃培养条件下,福氏志贺菌菌体蛋白和膜囊泡蛋白的鉴定总数分别为1 510、231、1 412、281,4种组分的共有蛋白数为156。生物信息学分析结果发现:膜囊泡中特异性富集了核糖体结构蛋白和膜起源蛋白,且37℃培养条件下的膜囊泡特异包被了大量三型分泌系统相关蛋白。【结论】运用比较蛋白质组学技术首次分析了不同温度下福氏志贺菌及其膜囊泡的蛋白质组变化,发现一些温度相关的膜囊泡特异包被蛋白,为深入研究膜囊泡的选择性包被奠定基础。
译  名：
Comparative Proteomic Analysis of Shigella flexneri and Its Outer Membrane Vesicle
作  者：
HAN Dongmei;WEI Candong;Institute of Pathogen Biology,Chinese Academy of Medical Sciences;
关键词：
Shigella flexneri;;outer membrane vesicle;;proteome;;T3SS
摘  要：
[Purpose] To gain a further understanding of the biological activity that Shigella flexneri exports via outer membrane vesicles( OMVS),we performed comparative proteomics analysis of protein profiles between bacteria and its outer membrane vesicles in response to temperature. [Method]Wildtype S. flexneir 2 a strain 301 and its outer membrane vesicle respectively grown at 37 ℃ and 30 ℃ were collected by sequential centrifugation. Furtherly,OMVs sample were purified by density gradient ultracentrifugation. Then the four samples were subject to shotgun proteomicanalysis by two-dimensional UPLC-linear ion trap mass spectrometry system. [Results]Total 1 510,231,281 and 1 412 proteins were detected in bacteria itself and outer membrane vesicle grown at 37 ℃ and 30 ℃ respectively. Of which,156 proteins were common in all four samples. Further bioinformatics analysis found that there were specifically rich in COG categories of ribosomal structure and cell membrane biogenesis in the outer membrane vesicles samples. Besides,OMVs derived from strains grown at 37 ℃ specifically packaged alarge number of Mxi-Spa type III secretion system effectors. [Conclusion] Utilizing comparative proteomics technology,this study analyzed the changes of proteome of S. flexneri and its OMVs grown at different temperature for the first time and found some unique temperature-related vesicular proteins. Additionally,those selective cargo proteins detected in OMVs could serve as the groundwork for further disclosing OMV's selective package.

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