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当前位置: 首页 > 文章 > 紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应 植物保护 2010,36 (5) 105-109
Position: Home > Articles > Mutagenetic effects of UV and He-Ne laser on the strain PT2 with antagonistic ability against Coniella diplodiella Plant Protection 2010,36 (5) 105-109

紫外线、He-Ne激光对葡萄白腐病菌拮抗菌PT2的复合诱变效应

作  者：
巩洁;陈亮;陈立;韩文霞;陈五岭
单  位：
西北大学生命科学学院
关键词：
紫外线;He-Ne激光;枯草芽胞杆菌;葡萄白腐病菌;复合诱变
摘  要：
采用紫外线、He-Ne激光及两者复合诱变的方法,分别处理对葡萄白腐病有拮抗作用的枯草芽胞杆菌PT2,以提高其抑菌效果。结果表明,紫外线和He-Ne激光单独作用,所筛菌株UV10与HN14抑菌效果提高率分别为15.0%与21.7%。而经过对复合诱变条件确定,在紫外线照射90 s后,再用15 mW的He-Ne激光辐照10 min,筛选出最佳正变菌株F15,抑菌效果提高30.9%,明显高于单因素诱变,经25代传代试验证明其拮抗能力可以进行稳定遗传。
译  名：
Mutagenetic effects of UV and He-Ne laser on the strain PT2 with antagonistic ability against Coniella diplodiella
作  者：
Gong Jie,Chen Liang,Chen Li,Han Wenxia,Chen Wuling(Institute of Life Sciences,Northwest University,Xi'an 710069,China)
关键词：
UV;He-Ne laser;Bacillus subtilis;Coniella diplodiella;compound mutation
摘  要：
The strain PT2 Bacillus subtilis,which has an antagonistic ability to Coniella diplodiella Petrak & Syd.was irradiated respectively by UV and He-Ne laser,and the mutant strains UV10 and HN14 were obtained.Both of them increased the antagonistic ability by 15.0% and 21.7% compared with PT2.The results indicated that the coordinative effect of UV and He-Ne laser was better than that by using them individually.The cells were treated firstly by UV for 90 seconds and then by He-Ne laser for 10 minutes in 15mW.The best positive mutation strain F15 was obtained,whose antagonistic ability was 30.9% higher than PT2's.By cultivating it from 1st to 25th generation,its antagonistic ability proved to be stable in heredity.

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