当前位置: 首页 > 文章 > 红豆越橘花色苷的分离及功能性研究 中国林副特产 2011 (5) 5-8
Position: Home > Articles > Separation and Functionalities of Anthocyanins from Vaccinium vitis-idaea Forest By-Product and Speciality in China 2011 (5) 5-8

红豆越橘花色苷的分离及功能性研究

作  者:
樊梓鸾;王振宇;左丽丽;田双起
单  位:
哈尔滨工业大学食品科学与工程学院
关键词:
红豆越橘;花色苷;抗氧化;抗细胞增殖
摘  要:
采用活性跟踪的方法分离纯化野生红豆越橘提取物,并将有效组分进行抗癌细胞增殖活性测定。首先采用大孔吸附树脂X-5富集分离红豆越橘丙酮提取物,多酚含量和ABTS抗氧化做指标确定乙醇梯度洗脱有效组分,并命名为LB-1、LB-2、LB-3、LB-4,其总洗脱物命名为LB-MIX。用MTT法检测以上5个组分抗结肠癌HT29、卵巢癌Hela、肺癌A549和肝癌HepG-2细胞增殖活性,并选用环磷酰胺做阳性对照。结果显示,LB-MIX具有较好的抑制几株癌细胞的增殖活性。
译  名:
Separation and Functionalities of Anthocyanins from Vaccinium vitis-idaea
作  者:
Fan Ziluan1,Wang Zhenyu1,2*,Zuo Lili1,Tian Shuangqi1(1.School of Food Science and Engineering,Harbin Institute of Technology,Harbin 150090; 2.School of Forestry,Northeast Forestry University,Harbin 150040)
关键词:
Vaccinium vitis-idaea;Anthocyanins;Antioxidant;Antiproliferation
摘  要:
In this experiment,purified extracts of Vaccinium vitis-idaea with the method of tracking activity,and determine the main anthocyanin composition by liquid chromatography-mass spectrometry techniques.First,enrichment and separation of acetone extracts of Vaccinium vitis-idaea by macroporous resin X-5,and determine the effective components of alcohol gradient by the phenolic contentation and scavenging activity of ABTS radical,and named LB-1,LB-2,LB-3,LB-4,the total eluate named LB-MIX.The inhibition of HT29 colon cancer,Hela cervical cancer,A549 lung cancer,and HepG-2 liver cancer cell proliferation of five fractions were employed by MTT assay.The results showed that LB-MIX has a better inhibition of the four cancer cells proliferation activity.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊