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Position: Home > Articles > Study on Extraction and Purification of Total Flavones from Bamboo Leaves FOOD SCIENCE 2005,26 (8) 158-161

竹叶总黄酮的提取和纯化工艺的研究

作  者:
朱宏莉;韦海洪;宋纪蓉 ;陈邦;任静
单  位:
西北大学生命科学学院,西北大学生命科学学院,西北大学化学系,西北大学生命科学学院 陕西西安710069,陕西西安710069,陕西西安710069,陕西西安710069;西北大学生命科学学院
关键词:
竹叶;总黄酮;提取;纯化
摘  要:
采用L9(34)正交试验设计,以溶剂种类、溶剂浓度、料液比、浸提时间,作为主要考虑因素,确定了竹叶黄酮的最佳提取工艺为:60%丙酮,料液比为1:40的条件下回流浸提3h。其中,溶剂浓度和料液比是两个主要影响因素,其p<0.01,达到了显著性水平。在此基础上,采用x-5大孔吸附树脂与聚酰胺树脂联用的方法纯化竹叶提取物,最终竹叶总黄酮含量可达78.97%。
译  名:
Study on Extraction and Purification of Total Flavones from Bamboo Leaves
作  者:
ZHU Hong-li1,WEI Hai-hong1,SONG Ji-rong*,CHEN Bang2,REN Jing1 (1.Institute of Life Science, Northwest University, Xi'an 710069, China; 2.Chemistry Department, Northwest University, Xi'an 710069, China)
关键词:
bamboo leaves;total flavones;extraction;purification
摘  要:
The optimum extracting process of total flavones from bamboo leaves was obtained after using L9(34) orthogonal test plan. We selected four elements——solvent type; solvent concentration; material-solution ratio and extracting time to observe their effects on the extraction of bamboo flavones. The orthogonal test results showed that the optimum conditions were: 60%, acetone, 3h, material-solution ratio 1: 40. The major factors affecting the content of total flavones were the solvent concentration and the material-solution ratio. Both of them had a significant effect on the yield (F>F0.01). On the basis of these experiments, we used x-5 macroreticuler resin together with polyamide to purify the extraction, and the final content of total flavones was up to 78.97%.

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