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Position: Home > Articles > RP-HPLC Determination of Rutin,Quercetin and Syringic Acid in Flammulina velutipe Fruitbody FOOD SCIENCE 2009,30 (18) 230-232

RP-HPLC法测定金针菇中芦丁、槲皮素和丁香酸的含量

作  者:
陈智毅;廖森泰;刘学铭;吴娱明;邹宇晓;施英
单  位:
广东省农业科学院蚕业与农产品加工研究所,广东省农产品加工公共实验室;广东省农业科学院蚕业与农产品加工研究所
关键词:
金针菇;芦丁;槲皮素;丁香酸;反相高效液相色谱法
摘  要:
采用高效液相色谱法测定金针菇中的多酚类成分,使用AgilentZORBAXSB-C18分析柱(4.6mm×250mm,5μm),流动相为乙腈(A)-0.4%乙酸(B),其中0~40min为5%A-95%B,40~45min为25%A-75%B,45~50min为35%A-65%B,之后运行5min,流速1.0ml/min,VWD检测器,检测波长280nm,柱温30℃。本研究在鲜金针菇中检出芦丁和槲皮素、在干金针菇中检出槲皮素和丁香酸。
译  名:
RP-HPLC Determination of Rutin,Quercetin and Syringic Acid in Flammulina velutipe Fruitbody
作  者:
CHEN Zhi-yi,LIAO Sen-tai,LIU Xue-ming,WU Yu-ming,ZOU Yu-xiao,SHI Ying (Guangdong Open Access Laboratory of Agricultural Product Processing, Institute of Sericulture and Farm Produce Processing Research, Guangdong Academy of Agricultural Sciences, Guangzhou 510610, China)
关键词:
Flammulina velutipe;rutin;quercetin;syringic acid;RP-HPLC
摘  要:
A HPLC method was established to determine polyphenolic compounds in Flammulina velutipe fruitbody. The chromatographic separation was achieved on an Agilent Zorbax SB-C18 (4.6 mm × 250 mm, 5 μm) analytical column using a mobile phase consisting of a mixture of A: acetonitrile and B: 0.4% acetic acid (0-40 min: 5% A and 95% B; 40-45 min: 25% A and 75% B; 45-50 min: 35% A and 65% B) at a flow rate of 1.0 ml/min and 30 ℃ column temperature , and the analytes were detected using a VWD detector at 280 nm wavelength. Rutin and quercetin both were found in fresh Flammulina velutipe fruitbody, while quercetin, together with syringic acid was detected in dried samples.

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