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当前位置: 首页 > 文章 > 雪莲果体外抗氧化和自由基清除能力 食品科学 2010,31 (17) 166-169
Position: Home > Articles > in vitro Antioxidant and Free Radical Scavenging Activities of Yacon (Smallanthus sonchifolius) Tubers FOOD SCIENCE 2010,31 (17) 166-169

雪莲果体外抗氧化和自由基清除能力

作  者：
杨少辉;宋英今;王洁华;季静
单  位：
天津大学农业与生物工程学院
关键词：
雪莲果;块根;抗氧化;自由基
摘  要：
抗氧化能力是衡量果蔬营养及保健价值的重要指标之一。雪莲果是一种药食两用植物,对多种慢性疾病有缓解作用。分别采用5种方法(DPPH、ABTS、FRAP、SASR和MCC)对雪莲果块根甲醇提取液的自由基清除能力及抗氧化活性进行体外评价。结果表明:DPPH法测定的Trolox当量抗氧化能力(TEAC)为410.263mgTrolox/gmd,抗坏血酸当量抗氧化能力(AEAC)为230.485mgVC/gmd,IC50值为1.464mg/mL;ABTS法测定的TEAC为267.584mg Trolox/gmd,AEAC为41.597mgVC/gmd,IC50值为1.269mg/mL;SRSA法测定的TEAC为652.816mg Trolox/gmd,AEAC为101.451mgVC/gmd,IC50值为7.720mg/mL。说明雪莲果块根提取物对DPPH自由基、ABTS+·和O2·三种不同的自由基均有一定的清除活性。此外,雪莲果块根提取物还具有较高的总抗氧化(FRAP值为131.723mgFeSO4/gmd)和较强的金属螯合能力(73.193%)。
译  名：
in vitro Antioxidant and Free Radical Scavenging Activities of Yacon (Smallanthus sonchifolius) Tubers
作  者：
YANG Shao-hui,SONG Ying-jin,WANG Jie-hua,JI Jing (School of Agriculture and Bioengineering,Tianjin University,Tianjin 300072,China)
关键词：
yacon;tuber;antioxidant activity;radical
摘  要：
Antioxidant capacity is an important factor indicating nutritional and health value of fruits and vegetables.Yacon is a both edible and medicinal plant,which has some preventing roles for a number of chronic diseases.In this study,to assess the in vitro antioxidant potential of yacon tubers,the 60% methanol extract from yacon tubers was tested for its scavenging capacities against DPPH,ABTS+·,superoxide anion radicals,ferric reducing antioxidant power and ferrous ions chelating capacity.The DPPH radical scavenging assay of the extract showed a trolox equivalent antioxidant capacity (TEAC) of 410.263 mg Trolox /g md,a ascorbic acid equivalent antioxidant capacity (AEAC) of 230.485 mg VC/g md and a median inhibition concentration (IC50) of 1.464 mg/mL,and the ABTS+· radical scavenging assay showed a TEAC of 267.584 mg Trolox/g md,an AEAC of 41.597 mg VC/g md and an IC50 of 1.269 mg/mL,and the TEAC,AEAC and IC50 obtained in the superoxide anion radical assay were 652.816 mg Trolox/g md,101.451 mg VC/g md and 7.720 mg/mL,respectively.These results demonstrate that the extract from yacon tubers has scavenging effect against all DPPH,ABTS+·,superoxide anion radicals.Moreover,the extract also presented high ferric reducing antioxidant power (131.723 mg FeSO4 /g md) and strong ferrous ions chelating capacity (73.193%).

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